肥胖和哮喘青年脂肪组织的免疫特征。

IF 2.7 3区医学 Q1 PEDIATRICS Pediatric Obesity Pub Date : 2023-10-04 DOI:10.1111/ijpo.13078

Anna Reichenbach, Wade O'Brien, Sarai Duran, Kayla J. Authelet, Robert J. Freishtat, Evan P. Nadler, Deepa Rastogi

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引用次数: 0

摘要

背景：肥胖是儿童哮喘的一个危险因素。肥胖介导的全身炎症与代谢失调相关；两者都与哮喘负担有关。然而，脂肪组织炎症在肥胖相关哮喘中并不明确。目的：明确儿童肥胖相关哮喘的脂肪组织炎症及其与代谢指标的关系。方法：来自肥胖相关哮喘青年（n = 14）和没有哮喘的肥胖（n = 23）进行流式细胞术分析，并与代谢指标相关。结果：与没有哮喘的青年相比，患有肥胖相关哮喘的青年的VAT富含白细胞和巨噬细胞，包括M1和双M1M2细胞，但CD4+淋巴细胞、内皮细胞、其祖细胞和前脂肪细胞没有差异。在没有哮喘的肥胖青年中，M1巨噬细胞计数与葡萄糖呈正相关，而M1M2细胞、CD4+淋巴细胞及其亚群与高密度脂蛋白呈负相关，但在肥胖相关哮喘青年中则不然。结论：肥胖相关哮喘青年VAT中巨噬细胞介导的炎症富集支持其在与哮喘发病率相关的全身炎症中的作用。在患有肥胖相关哮喘的青年中，VAT细胞与代谢失调缺乏相关性，这表明有必要确定肥胖相关哮喘中与VAT炎症相关的区别因素。

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Immune profile of adipose tissue from youth with obesity and asthma

Background

Obesity is a risk factor for paediatric asthma. Obesity-mediated systemic inflammation correlates with metabolic dysregulation; both are associated with asthma burden. However, adipose tissue inflammation is not defined in obesity-related asthma.

Objective

Define adipose tissue inflammation and its association with metabolic measures in paediatric obesity-related asthma.

Methods

Cellular profile of stromal vascular fraction from visceral adipose tissue (VAT) from youth with obesity-related asthma (n = 14) and obesity without asthma (n = 23) was analyzed using flow cytometry and correlated with metabolic measures.

Results

Compared to youth without asthma, VAT from youth with obesity-related asthma was enriched for leukocytes and macrophages, including M1 and dual M1M2 cells, but did not differ for CD4⁺ lymphocytes, and endothelial cells, their progenitors, and preadipocytes. M1 macrophage counts positively correlated with glucose, while M1M2 cells, CD4⁺ lymphocytes, and their subsets negatively correlated with high-density lipoprotein, in youth with obesity without asthma, but not among those with obesity-related asthma.

Conclusions

Enrichment of macrophage-mediated inflammation in VAT from youth with obesity-related asthma supports its role in systemic inflammation linked with asthma morbidity. Lack of correlation of VAT cells with metabolic dysregulation in youth with obesity-related asthma identifies a need to define distinguishing factors associated with VAT inflammation in obesity-related asthma.

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来源期刊

Pediatric Obesity PEDIATRICS-

CiteScore

7.30

自引率

5.30%

发文量

117

审稿时长

6-12 weeks

期刊介绍： Pediatric Obesity is a peer-reviewed, monthly journal devoted to research into obesity during childhood and adolescence. The topic is currently at the centre of intense interest in the scientific community, and is of increasing concern to health policy-makers and the public at large. Pediatric Obesity has established itself as the leading journal for high quality papers in this field, including, but not limited to, the following: Genetic, molecular, biochemical and physiological aspects of obesity – basic, applied and clinical studies relating to mechanisms of the development of obesity throughout the life course and the consequent effects of obesity on health outcomes Metabolic consequences of child and adolescent obesity Epidemiological and population-based studies of child and adolescent overweight and obesity Measurement and diagnostic issues in assessing child and adolescent adiposity, physical activity and nutrition Clinical management of children and adolescents with obesity including studies of treatment and prevention Co-morbidities linked to child and adolescent obesity – mechanisms, assessment, and treatment Life-cycle factors eg familial, intrauterine and developmental aspects of child and adolescent obesity Nutrition security and the "double burden" of obesity and malnutrition Health promotion strategies around the issues of obesity, nutrition and physical activity in children and adolescents Community and public health measures to prevent overweight and obesity in children and adolescents.