{"title":"莳萝(Anethum Graveolens)空中部分溶剂提取物的生物测定指导分离及其生物效应","authors":"Nichakan Peerakam, Sanit Thongnest, Prapart Phoowiang, Kwanchayanawish Machana","doi":"10.1080/10496475.2021.1989734","DOIUrl":null,"url":null,"abstract":"ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Bioassay Guided-isolation of Phytochemicals and Their Biological Effects of Solvent Extracts of Dill (Anethum Graveolens) Aerial Portions\",\"authors\":\"Nichakan Peerakam, Sanit Thongnest, Prapart Phoowiang, Kwanchayanawish Machana\",\"doi\":\"10.1080/10496475.2021.1989734\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.\",\"PeriodicalId\":35803,\"journal\":{\"name\":\"Journal of Herbs, Spices and Medicinal Plants\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2021-11-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbs, Spices and Medicinal Plants\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10496475.2021.1989734\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbs, Spices and Medicinal Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10496475.2021.1989734","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Bioassay Guided-isolation of Phytochemicals and Their Biological Effects of Solvent Extracts of Dill (Anethum Graveolens) Aerial Portions
ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.
期刊介绍：
The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.
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