激光烧蚀法合成荧光碳点在生物成像中的应用

J. Jumardin, A. Maddu, Kokoeh Santoso, I. Isnaeni
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引用次数: 0

摘要

通过将激光束聚焦在胶体(CH3)中的碳(Tea)材料上3小时,使用激光烧蚀合成碳点(CDs)。UV-Vis光谱和荧光表征显示了由对照处理以及使用聚乙二醇(PEG400)进行激光烧蚀和涂覆之后引起的波长峰值的吸收。激发能和发射能是CDs吸收波长和荧光强度的公式。吸光度系数是基于比色杯厚度的吸光度值而获得的。透射率值(T)是基于吸收系数乘以100%而获得的。基于对照参数的CD荧光波长为489nm。激光烧蚀后为496nm,涂覆后为511nm。基于TEM测量,CD的形态和尺寸特征为4nm至10nm。荧光分析在斑马鱼眼睛器官内内化蓝CD发光强度值的生物成像应用。眼器官、鳃、肠、背侧和尾部注射点的CD平均强度分别为88.15%、91.58%、92.76%和0.00%。
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Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application
Carbon Dots (CDs) were synthesized using laser ablation by focusing the laser beam on carbon (Tea) material in colloid (CH3) for 3 hours. UV-Vis spectroscopic and fluorometric characterization showed absorption of the wavelength peaks caused by the control treatment and after laser ablation and coating using Poly Ethylene Glycol (PEG400). The excitation and emission energies are formulations of CDs absorbance wavelength and fluorescence intensity. The absorbance coefficient is obtained based on the absorbance value of the cuvette thickness. The transmittance value (T) is obtained based on the absorption coefficient multiplied by 100%. CD fluorescence wavelength based on control parameters was 489 nm. After laser ablation was 496 nm, and after coating was 511 nm. CDs morphology and size characteristics are 4 nm to 10 nm based on TEM measurements. Fluorescence analysis for bioimaging applications on the luminescence intensity value of internalized blue CDs in zebrafish eye organs. The average intensity of CDs in the eye organs, gill, intestinal, dorsal, and tail injection points was 88.15 %, 91.58 %, 92.76 %, and 0.00 %.
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