Fabrication and Bioapplications of Magnetically Modified Chitosan-based Electrospun Nanofibers

Abstract The fabrication of magnetically modified electrospun nanocomposite fibers based on a naturally-derived biocompatible and biodegradable polysaccharide chitosan (CS) and the hydrophilic and biocompatible poly(vinylpyrrolidone) (PVP) is reported herein. The anchoring of magnetic nanoparticles (MNPs) onto the surfaces of the electrospun PVP/CS fibers was carried out by a post-magnetization process based on chemical coprecipitation, via immersing the produced fibrous mats in an aqueous solution containing Fe(II) and Fe(III) salts at appropriate molar ratios, followed by the addition of a weak base to yield MNPs. Electron microscopy revealed the presence of continuous micron and submicron fibers surface-decorated with MNPs. The magnetically modified PVP/CS fibers exhibited superparamagnetic behavior at ambient temperature. The magnetic fibrous nanocomposite carrier was employed for the immobilization of Saccharomyces cerevisiae cells and their use for sucrose hydrolysis, and Candida rugosa lipase and its use for artificial substrate hydrolysis.