Suyoun Choi, Yukyung Choi, Yeongeun Seo, Soomin Lee, Yohan Yoon
{"title":"单核细胞增生李斯特菌mfm2019‐FV16全基因组测序鉴定致病性相关遗传变异","authors":"Suyoun Choi, Yukyung Choi, Yeongeun Seo, Soomin Lee, Yohan Yoon","doi":"10.1111/jfs.13076","DOIUrl":null,"url":null,"abstract":"<p>In this study, the characteristics of <i>Listeria monocytogenes</i> isolated from enoki mushroom (<i>Flammulina velutipes</i>) collected from May to July 2019 were analyzed. The isolates were analyzed for strain characteristics, hemolysis, antibiotic resistance, heat resistance, and virulence genes (<i>inlA</i>, <i>inlB</i>, <i>actA</i>, <i>plcB</i>, and <i>hlyA</i>) were analyzed, and whole genome sequencing (WGS) analysis was performed for the strain with the highest risk. Seven isolates were obtained from some enoki mushroom samples. All isolates were β-hemolytic and had virulence genes. Antibiotic resistance test showed multi-drug resistance in <i>L</i>. <i>monocytogenes</i> strain SMFM2019-FV15 and SMFM2019-FV16. <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 showed higher heat resistance than the other isolates. According to the results <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 was selected as the strain with the highest risk. WGS for <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 showed that it had 14 antibiotic resistance genes and 43 virulence genes, and 35 single nucleotide variants in the 43 virulence genes were observed. These results indicate that these genetic variations might be related to the high risk of <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16.</p>","PeriodicalId":15814,"journal":{"name":"Journal of Food Safety","volume":"43 6","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of genetic variations related to pathogenicity by whole genome sequencing of Listeria monocytogenes SMFM2019-FV16 isolated from enoki mushroom\",\"authors\":\"Suyoun Choi, Yukyung Choi, Yeongeun Seo, Soomin Lee, Yohan Yoon\",\"doi\":\"10.1111/jfs.13076\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>In this study, the characteristics of <i>Listeria monocytogenes</i> isolated from enoki mushroom (<i>Flammulina velutipes</i>) collected from May to July 2019 were analyzed. The isolates were analyzed for strain characteristics, hemolysis, antibiotic resistance, heat resistance, and virulence genes (<i>inlA</i>, <i>inlB</i>, <i>actA</i>, <i>plcB</i>, and <i>hlyA</i>) were analyzed, and whole genome sequencing (WGS) analysis was performed for the strain with the highest risk. Seven isolates were obtained from some enoki mushroom samples. All isolates were β-hemolytic and had virulence genes. Antibiotic resistance test showed multi-drug resistance in <i>L</i>. <i>monocytogenes</i> strain SMFM2019-FV15 and SMFM2019-FV16. <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 showed higher heat resistance than the other isolates. According to the results <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 was selected as the strain with the highest risk. WGS for <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16 showed that it had 14 antibiotic resistance genes and 43 virulence genes, and 35 single nucleotide variants in the 43 virulence genes were observed. These results indicate that these genetic variations might be related to the high risk of <i>L</i>. <i>monocytogenes</i> SMFM2019-FV16.</p>\",\"PeriodicalId\":15814,\"journal\":{\"name\":\"Journal of Food Safety\",\"volume\":\"43 6\",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Safety\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13076\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Safety","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jfs.13076","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Identification of genetic variations related to pathogenicity by whole genome sequencing of Listeria monocytogenes SMFM2019-FV16 isolated from enoki mushroom
In this study, the characteristics of Listeria monocytogenes isolated from enoki mushroom (Flammulina velutipes) collected from May to July 2019 were analyzed. The isolates were analyzed for strain characteristics, hemolysis, antibiotic resistance, heat resistance, and virulence genes (inlA, inlB, actA, plcB, and hlyA) were analyzed, and whole genome sequencing (WGS) analysis was performed for the strain with the highest risk. Seven isolates were obtained from some enoki mushroom samples. All isolates were β-hemolytic and had virulence genes. Antibiotic resistance test showed multi-drug resistance in L. monocytogenes strain SMFM2019-FV15 and SMFM2019-FV16. L. monocytogenes SMFM2019-FV16 showed higher heat resistance than the other isolates. According to the results L. monocytogenes SMFM2019-FV16 was selected as the strain with the highest risk. WGS for L. monocytogenes SMFM2019-FV16 showed that it had 14 antibiotic resistance genes and 43 virulence genes, and 35 single nucleotide variants in the 43 virulence genes were observed. These results indicate that these genetic variations might be related to the high risk of L. monocytogenes SMFM2019-FV16.
期刊介绍：
The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.
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