肥胖患者减肥手术后脂肪组织、肝组织和骨骼肌中枢基因及其与免疫浸润的关联分析

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2022-04-12 DOI:10.1080/21623945.2022.2060059
Kemin Yan, Pengyuan Zhang, Jiewen Jin, Xin Chen, H. Guan, Yanbing Li, Hai Li
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引用次数: 2

摘要

减肥手术(BS)是治疗肥胖的有效方法。脂肪组织、肝组织和骨骼肌是重要的代谢组织。本研究利用Gene Expression Omnibus数据集,通过生物信息学分析肥胖症患者BS后这些代谢组织中的枢纽基因及其与免疫浸润的关系。鉴定差异表达基因(DEGs),构建蛋白互作网络鉴定枢纽基因。结果鉴定出121个常见的DEGs,主要富集于细胞因子-细胞因子受体相互作用、趋化因子信号通路、中性粒细胞活化和免疫应答。免疫细胞浸润分析显示,BS后脂肪组织和肝脏组织中M1巨噬细胞丰度显著降低(p<0.05)。10个中心基因(TYROBP、TLR8、FGR、NCF2、HCK、CCL2、LAPTM5、MNDA和S100A9)在BS后均下调,也与免疫细胞相关。验证数据集中的结果一致显示,肥胖患者和小鼠中这些枢纽基因的表达水平升高,BS后表达水平下降。综上所述,本研究分析了肥胖患者三个关键代谢组织中BS的潜在免疫和炎症机制,揭示了与免疫细胞浸润相关的枢纽基因,为肥胖治疗提供了潜在靶点。
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Integrative analyses of hub genes and their association with immune infiltration in adipose tissue, liver tissue and skeletal muscle of obese patients after bariatric surgery
ABSTRACT Bariatric surgery (BS) is an effective treatment for obesity. Adipose tissue, liver tissue and skeletal muscle are important metabolic tissues. This study investigated hub genes and their association with immune infiltration in these metabolic tissues of obese patients after BS by bioinformatic analysis with Gene Expression Omnibus datasets. Differentially expressed genes (DEGs) were identified, and a protein–protein interaction network was constructed to identify hub genes. As a result, 121 common DEGs were identified and mainly enriched in cytokine–cytokine receptor interactions, chemokine signaling pathway, neutrophil activation and immune responses. Immune cell infiltration analysis showed that the abundance of M1 macrophages was significantly lower in adipose and liver tissue after BS (p<0.05). Ten hub genes (TYROBP, TLR8, FGR, NCF2, HCK, CCL2, LAPTM5, MNDA and S100A9) that were all downregulated after BS were also associated with immune cells. Consistently, results in the validated dataset showed that the expression levels of these hub genes were increased in obese patients and mice, and decreased after BS. In conclusion, this study analysed the potential immune and inflammatory mechanisms of BS in three key metabolic tissues of obese patients, and revealed hub genes associated with immune cell infiltration, thus providing potential targets for obesity treatment.
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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
期刊最新文献
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