肠道病毒在干燥表面的稳定性和感染性:间接传播控制的潜力

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Biosafety and Health Pub Date : 2023-12-01 DOI:10.1016/j.bsheal.2023.07.001
Pichamon Sittikul , Pimolpachr Sriburin , Jittraporn Rattanamahaphoom , Warisa Nuprasert , Nipa Thammasonthijarern , Janjira Thaipadungpanit , Weerawan Hattasingh , Nathamon Kosoltanapiwat , Pilaipan Puthavathana , Supawat Chatchen
{"title":"肠道病毒在干燥表面的稳定性和感染性:间接传播控制的潜力","authors":"Pichamon Sittikul ,&nbsp;Pimolpachr Sriburin ,&nbsp;Jittraporn Rattanamahaphoom ,&nbsp;Warisa Nuprasert ,&nbsp;Nipa Thammasonthijarern ,&nbsp;Janjira Thaipadungpanit ,&nbsp;Weerawan Hattasingh ,&nbsp;Nathamon Kosoltanapiwat ,&nbsp;Pilaipan Puthavathana ,&nbsp;Supawat Chatchen","doi":"10.1016/j.bsheal.2023.07.001","DOIUrl":null,"url":null,"abstract":"<div><p>Hand, foot, and mouth disease (HFMD) is a contagious disease mainly occurring in young children, and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand. Moreover, the World Health Organization (WHO) monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System. HFMD is mainly caused by a group of enteroviruses (EVs) transmitted through direct contact (person to person) and indirect contact with contaminated objects (surface-to-hand). However, few studies have examined the surface stability of EVs. In this study, we investigated the stability of enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) on three different dry surfaces (wood, plastic, and stainless steel) using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) staining of viable cells and real-time polymerase chain reaction (viral genome detection). The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces. However, viral RNA could be detected on dry surfaces for up to 28 days. Concerning heat inactivation, both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min. Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.</p></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"5 6","pages":"Pages 339-345"},"PeriodicalIF":3.5000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590053623000794/pdfft?md5=aac47793878925a98a1ea15c3c47d054&pid=1-s2.0-S2590053623000794-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Stability and infectivity of enteroviruses on dry surfaces: Potential for indirect transmission control\",\"authors\":\"Pichamon Sittikul ,&nbsp;Pimolpachr Sriburin ,&nbsp;Jittraporn Rattanamahaphoom ,&nbsp;Warisa Nuprasert ,&nbsp;Nipa Thammasonthijarern ,&nbsp;Janjira Thaipadungpanit ,&nbsp;Weerawan Hattasingh ,&nbsp;Nathamon Kosoltanapiwat ,&nbsp;Pilaipan Puthavathana ,&nbsp;Supawat Chatchen\",\"doi\":\"10.1016/j.bsheal.2023.07.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Hand, foot, and mouth disease (HFMD) is a contagious disease mainly occurring in young children, and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand. Moreover, the World Health Organization (WHO) monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System. HFMD is mainly caused by a group of enteroviruses (EVs) transmitted through direct contact (person to person) and indirect contact with contaminated objects (surface-to-hand). However, few studies have examined the surface stability of EVs. In this study, we investigated the stability of enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) on three different dry surfaces (wood, plastic, and stainless steel) using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) staining of viable cells and real-time polymerase chain reaction (viral genome detection). The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces. However, viral RNA could be detected on dry surfaces for up to 28 days. Concerning heat inactivation, both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min. Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.</p></div>\",\"PeriodicalId\":36178,\"journal\":{\"name\":\"Biosafety and Health\",\"volume\":\"5 6\",\"pages\":\"Pages 339-345\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2590053623000794/pdfft?md5=aac47793878925a98a1ea15c3c47d054&pid=1-s2.0-S2590053623000794-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosafety and Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590053623000794\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosafety and Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590053623000794","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
引用次数: 0

摘要

手足口病是一种主要发生在幼儿身上的传染性疾病,在包括泰国在内的亚太地区,幼儿中经常爆发手足口病。此外,世界卫生组织(WHO)通过区域事件监测系统对西太平洋地区的手足口病进行监测,以发现疫情爆发和其他重大事件。手足口病主要由一组肠道病毒(EVs)引起,通过直接接触(人与人之间)和间接接触受污染物体(手与手之间)传播。然而,很少有研究探讨肠道病毒的表面稳定性。在本研究中,我们使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-溴化四氮唑(MTT)染色法和实时聚合酶链反应(病毒基因组检测)研究了肠病毒 A71(EV-A71)和柯萨奇病毒 A16(CVA16)在三种不同干燥表面(木材、塑料和不锈钢)上的稳定性。结果表明,在干燥的表面上,病毒的感染力在几小时内急剧下降。不过,在干燥表面上检测病毒 RNA 的时间可长达 28 天。关于热灭活,EV-A71 和 CVA16 在暴露于 60°C 15 分钟后均失活。有关病毒在不同干燥表面稳定性的信息将为手足口病传播控制提供有用信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Stability and infectivity of enteroviruses on dry surfaces: Potential for indirect transmission control

Hand, foot, and mouth disease (HFMD) is a contagious disease mainly occurring in young children, and outbreaks commonly occur among young children in the Asia–Pacific region including Thailand. Moreover, the World Health Organization (WHO) monitors HFMD in the Western Pacific region to detect outbreaks and other significant events by the Regional Event Based Surveillance System. HFMD is mainly caused by a group of enteroviruses (EVs) transmitted through direct contact (person to person) and indirect contact with contaminated objects (surface-to-hand). However, few studies have examined the surface stability of EVs. In this study, we investigated the stability of enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) on three different dry surfaces (wood, plastic, and stainless steel) using the endpoint titration using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) staining of viable cells and real-time polymerase chain reaction (viral genome detection). The results revealed that virus infectivity dramatically decreased within a few hours on dry surfaces. However, viral RNA could be detected on dry surfaces for up to 28 days. Concerning heat inactivation, both EV-A71 and CVA16 were inactivated after exposure to 60°C for 15 min. Information on virus stability on different dry surfaces will provide useful information for HFMD transmission control.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
期刊最新文献
Biosafety and immunology: An interdisciplinary field for health priority Advances and challenges of mpox detection technology Construction of pseudotyped human coronaviruses and detection of pre-existing antibodies in the human population Vaccinia virus Tiantan strain blocks host antiviral innate immunity and programmed cell death by disrupting gene expression An outbreak of rhinovirus infection in a primary school in Shenyang City, China, in 2022
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1