拟除虫菊酯暴露的成年雄性Wistar大鼠血清炎症生物标志物水平、血糖控制指标和瘦素受体表达

Q3 Biochemistry, Genetics and Molecular Biology Journal of Cellular Biotechnology Pub Date : 2021-08-06 DOI:10.3233/jcb-210034
A. Atere, Akinpelu Moronkeji, Adebimpe Iyanuoluwa Moronkeji, H. Osadolor
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引用次数: 1

摘要

背景:由于农药的广泛使用,个体终生暴露于农药混合物中是不可避免的,导致毒性效应的增强,如由于暴露个体中自由基的形成而暂时或永久地改变代谢信号。目的:本研究随后观察了接触拟除虫菊酯的Wistar大鼠瘦素和胰岛素受体的表达及其对脂肪因子和血糖指数的影响。方法:随机选取Wistar大鼠84只,分为两组。51只大鼠暴露于1.2% w/v拟除虫菊酯类杀虫剂,其余33只大鼠未暴露。这些组被分为三个不同的组,每个组分别有7天、21天和41天。这些动物在麻醉下被处死,并在暴露数天后采集血液样本。测定总抗氧化状态(TAS)、丙二醛(MDA)、谷胱甘肽(GSH)、过氧化氢(H2O2)、一氧化氮(NO)、葡萄糖和脂质水平。测定血中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)、过氧化氢酶(CAT)的活性。ELISA法检测瘦素、脂联素、胰岛素、CRP、IL-6、TNFα。适当计算血糖指数。采用逆转录聚合酶链反应(RT-PCR)法检测瘦素和胰岛素的表达。所得数据进行统计学比较,P≤0.05被认为是显著的。结果:各暴露组TChol、TAG、HDL-C均值差异有统计学意义(p < 0.05)。暴露组血清SOD、GPx、CAT、GSH、TAS、QUICKI、TNFα、IL6、CRP平均水平显著降低(p < 0.05), MDA、H2O2、NO、胰岛素、HOMA-IR、FIRI、瘦素、脂联素平均水平显著升高(p < 0.05)。暴露组和非暴露组胰岛素和瘦素基因的相对表达量也用柱状图表示。结论:拟除虫菊酯类化学物质暴露可能通过增加暴露人群的肥胖风险而破坏碳水化合物和脂质代谢,这是不言而喻的。
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Serum levels of inflammatory biomarkers, glycaemic control indices and leptin receptors expression in adult male Wistar rats exposed to Pyrethroids
BACKGROUND: Due to widespread use of pesticides, an individual’s lifetime exposure to pesticide mixtures is unavoidable, resulting in potentiation of toxic effects such as changing metabolic signaling temporarily or permanently due to the formation of free radicals among exposed individuals. OBJECTIVE: This study then looked at the expression of leptin and insulin receptors in Wistar rats exposed to pyrethroid and its effects on adipokines and glycemic indices. METHODS: 84 Wistar rats were randomly selected and divided into two groups. 51 rats were exposed to 1.2 % w/v pyrethroid insecticides, while the remaining 33 rats were grouped as non-exposed. The groups were divided into three different groups, each with 7, 21, and 41 days. The animals were anesthetically sacrificed and samples of blood were collected after days of exposure. The levels of total antioxidant status (TAS), malondialdehyde (MDA), glutathione (GSH), hydrogen peroxide (H2O2), nitric oxide (NO), glucose and lipids were determined. Blood activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were also determined. ELISA was used to assess leptin, adiponectin, insulin, CRP, IL-6, and TNFα. Glycaemic indices were calculated appropriately. Leptin and Insulin expression was determined using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Data obtained were statistically compared, and P≤0.05 was considered significant. RESULTS: The mean TChol, TAG, and HDL-C were significantly different (p < 0.05) among the exposed groups. The serum mean levels of SOD, GPx, CAT, GSH, TAS, QUICKI, TNFα, IL6, and CRP were significantly reduced (p < 0.05), while mean levels of MDA, H2O2, NO, insulin, HOMA-IR, FIRI, leptin, and adiponectin were significantly elevated (p < 0.05) in exposed groups. The relative expression of insulin and leptin genes in exposed and non-exposed groups was also shown by column. CONCLUSION: It is self-evident that pyrethroid chemicals exposure may disrupt carbohydrate and lipid metabolism by raising the risk of obesity among the exposed groups.
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Journal of Cellular Biotechnology
Journal of Cellular Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
0.70
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发文量
13
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