M. Mantskava, Nugzar Pargalava, Ina Sherozia, N. Momtselidze, Giorgi Kuchava, Friedrich Jung, Lukas Plantl
BACKGROUND: Limb-threatening ischemia is one of more important problem of fundamental and practice modern angiology. Despite the development of modern diagnostic and therapeutic approaches good results, Limb-Threatening Ischemia deserves special attention and occupies a special place among medical, biomedical and socio-economic problems. A comprehensive study will make it possible to develop new approaches, the result of which should be a decrease in the total number of diseases, as well as a minimization of complications leading to large-scale tissue losses and amputations. OBJECTIVE: We set out to develop a new non-invasive method for studying microcirculation in patients with limb-threatening ischemia. METHODS: We investigated control group and adults of both sexes (age: 50–75 years) with a confirmed diagnosis of the claudication. Men and women in ratio 1 : 1 in control and target groups. RESULTS: The research results showed that the original microcirculation coefficient calculated according to our algorithm in patients with intermittent claudication was 70% better than in the control group and 120% of patients were worse than in patients with Limb-Threatening Ischemia. CONCLUSION: Our research team created an original so called coefficient of microcirculation. In order to determine it, we studied the blood flow in the pulsations on the left and right side of the posterior tibial arteries of the legs at rest (names of lines SIN F, DEX F) and during post-ischemic hyperemia in the same lines. The study of microcirculation based on the microcirculation coefficient is very informative. The importance of this methodology for studying the total peripheral blood flow and arteriolar response is due to fundamental and clinical developments, as well as financial costs. Considering the technique described in the article can be easily mastered by medical personnel, it is easy to use in the inpatient and outpatient sectors, its use does not require large areas of the medical facility, the equipment is not expensive and the test itself does not cause inconvenience to the patient, then we can recommend including the described method is included in the list of functional diagnostic tests as a direct and quantitative method for determining microcirculation (without special costs on the part of medical institutions, insurance companies and the patient).
背景：濒危肢体缺血是现代血管病学基础和实践中最重要的问题之一。尽管现代诊断和治疗方法的发展取得了良好的效果，但濒危肢体缺血仍值得特别关注，并在医学、生物医学和社会经济问题中占有特殊的地位。全面的研究将使开发新方法成为可能，其结果应该是减少疾病的总数，以及最大限度地减少导致大规模组织损失和截肢的并发症。目的：我们打算开发一种新的无创方法，用于研究肢体缺血患者的微循环。方法：我们调查了对照组和确诊为跛行的成年男女（年龄：50-75 岁）。男女比例为 1 ：1 的比例。结果：研究结果显示，根据我们的算法计算出的间歇性跛行患者的原始微循环系数比对照组好 70%，比肢体危重缺血患者差 120%。结论：我们的研究团队独创了一种所谓的微循环系数。为了确定该系数，我们研究了腿部胫后动脉左侧和右侧搏动的血流量，包括静息状态下的血流量（SIN F 和 DEX F 线的名称）和缺血后充血状态下的血流量。基于微循环系数的微循环研究信息量非常大。这种研究外周血流总量和动脉反应的方法之所以重要，是因为其基础和临床发展以及经济成本。考虑到文章中描述的技术医务人员很容易掌握，在住院和门诊都很容易使用，其使用不需要大面积的医疗设施，设备也不昂贵，测试本身也不会给病人带来不便，那么我们可以建议将描述的方法列入功能诊断测试清单，作为确定微循环的直接定量方法（医疗机构、保险公司和病人无需支付特殊费用）。
{"title":"Some questions about a new study of coefficient microcirculation in example in patients with limb-threatening ischemia and intermittent claudication","authors":"M. Mantskava, Nugzar Pargalava, Ina Sherozia, N. Momtselidze, Giorgi Kuchava, Friedrich Jung, Lukas Plantl","doi":"10.3233/jcb-249002","DOIUrl":"https://doi.org/10.3233/jcb-249002","url":null,"abstract":"BACKGROUND: Limb-threatening ischemia is one of more important problem of fundamental and practice modern angiology. Despite the development of modern diagnostic and therapeutic approaches good results, Limb-Threatening Ischemia deserves special attention and occupies a special place among medical, biomedical and socio-economic problems. A comprehensive study will make it possible to develop new approaches, the result of which should be a decrease in the total number of diseases, as well as a minimization of complications leading to large-scale tissue losses and amputations. OBJECTIVE: We set out to develop a new non-invasive method for studying microcirculation in patients with limb-threatening ischemia. METHODS: We investigated control group and adults of both sexes (age: 50–75 years) with a confirmed diagnosis of the claudication. Men and women in ratio 1 : 1 in control and target groups. RESULTS: The research results showed that the original microcirculation coefficient calculated according to our algorithm in patients with intermittent claudication was 70% better than in the control group and 120% of patients were worse than in patients with Limb-Threatening Ischemia. CONCLUSION: Our research team created an original so called coefficient of microcirculation. In order to determine it, we studied the blood flow in the pulsations on the left and right side of the posterior tibial arteries of the legs at rest (names of lines SIN F, DEX F) and during post-ischemic hyperemia in the same lines. The study of microcirculation based on the microcirculation coefficient is very informative. The importance of this methodology for studying the total peripheral blood flow and arteriolar response is due to fundamental and clinical developments, as well as financial costs. Considering the technique described in the article can be easily mastered by medical personnel, it is easy to use in the inpatient and outpatient sectors, its use does not require large areas of the medical facility, the equipment is not expensive and the test itself does not cause inconvenience to the patient, then we can recommend including the described method is included in the list of functional diagnostic tests as a direct and quantitative method for determining microcirculation (without special costs on the part of medical institutions, insurance companies and the patient).","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141825260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Liver is a vital organ and the role of Gamma aminobutyric acid (GABA) conjugated chitosan nanoparticles in enhancing the hepatocyte proliferation was reported. To understand the influence of these nanoparticles on various biochemical parameters during enhanced liver regeneration will improve its clinical significance. OBJECTIVE: To highlight the influence of GABA chitosan nanoparticles on ascorbic acid, hydroxyproline and lipid peroxidation levels during liver regeneration. METHODS: Intraperitoneal administration of nanoparticles was done to partially hepatectomised female Sprague Dawley rats (GCNP) and compared the biochemical parameters with sham operated control (C) and with no treatment (PHNT) cases. RESULTS: The hydroxyproline content was increased in the liver of GCNP when compared to PHNT (P < 0.05). The lipid peroxidation level was increased (P < 0.01) in PHNT compared to the control whereas, decreased in GCNP when compared (P < 0.01) with PHNT. There was a significant increase (P < 0.01) in the ascorbic acid content in PHNT when compared with C. It was significantly decreased (P < 0.01) in GCNP when compared with PHNT. CONCLUSION: This highlighted the therapeutic implications of lipid peroxidation, hydroxyproline and ascorbic acid in GABA chitosan nanoparticles mediated liver regeneration, which will have immense clinical relevance in maintaining liver health.
{"title":"Functional regulation of hydroxyproline, ascorbic acid and lipid peroxidation during GABA chitosan nanoparticles mediated liver regeneration","authors":"J. Shilpa, G.T. Nebu","doi":"10.3233/jcb-230129","DOIUrl":"https://doi.org/10.3233/jcb-230129","url":null,"abstract":"BACKGROUND: Liver is a vital organ and the role of Gamma aminobutyric acid (GABA) conjugated chitosan nanoparticles in enhancing the hepatocyte proliferation was reported. To understand the influence of these nanoparticles on various biochemical parameters during enhanced liver regeneration will improve its clinical significance. OBJECTIVE: To highlight the influence of GABA chitosan nanoparticles on ascorbic acid, hydroxyproline and lipid peroxidation levels during liver regeneration. METHODS: Intraperitoneal administration of nanoparticles was done to partially hepatectomised female Sprague Dawley rats (GCNP) and compared the biochemical parameters with sham operated control (C) and with no treatment (PHNT) cases. RESULTS: The hydroxyproline content was increased in the liver of GCNP when compared to PHNT (P < 0.05). The lipid peroxidation level was increased (P < 0.01) in PHNT compared to the control whereas, decreased in GCNP when compared (P < 0.01) with PHNT. There was a significant increase (P < 0.01) in the ascorbic acid content in PHNT when compared with C. It was significantly decreased (P < 0.01) in GCNP when compared with PHNT. CONCLUSION: This highlighted the therapeutic implications of lipid peroxidation, hydroxyproline and ascorbic acid in GABA chitosan nanoparticles mediated liver regeneration, which will have immense clinical relevance in maintaining liver health.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141665649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shivani Sachdeva, H. Saluja, Monica J. Mahajani, Vandana Tripathi, Aparna Deo, Amit Mani
Bone resorption is a feature of both osteoporosis and periodontitis. While, periodontitis causes localized inflammatory bone loss after an infected breach of the alveolar cortical bone and may cause tooth loss, osteoporosis causes systemic degenerative bone loss that results in loss of skeletal cancellous microstructure and eventual fracture. The majority of cross-sectional investigations have predominantly used radiographic data and to a lesser extent clinical criteria to confirm the link between osteoporosis and periodontitis. Age, heredity, hormonal changes, smoking, and a lack of calcium and vitamin D are just a few of the many common risk factors. Both disorders may have mutual effects and may even be risk factors for one another, necessitating concurrent therapy. Disruption of the homeostasis of bone remodelling, hormone balance, and inflammation resolution are proposed processes underpinning the relationship. Emerging is a mutual interventional strategy with intricate therapeutic interactions. Both disorders urge future well-controlled longitudinal and interventional studies for evidence-based clinical guidelines and call for interdisciplinary approaches to prevention and care.
骨吸收是骨质疏松症和牙周炎的共同特征。牙周炎会导致牙槽皮质骨感染破损后出现局部炎性骨质流失，并可能导致牙齿脱落，而骨质疏松症则会导致全身退行性骨质流失，造成骨骼松质骨微结构丧失，最终导致骨折。大多数横断面调查主要使用放射学数据，其次是临床标准来证实骨质疏松症与牙周炎之间的联系。年龄、遗传、荷尔蒙变化、吸烟、缺乏钙和维生素 D 只是众多常见风险因素中的几个。这两种疾病可能相互影响，甚至可能成为彼此的危险因素，因此有必要同时进行治疗。骨重塑、激素平衡和炎症消解的平衡被破坏，这些过程被认为是两者关系的基础。新出现的是一种具有复杂治疗相互作用的相互干预策略。这两种疾病都需要在未来进行良好控制的纵向和干预研究，以制定循证临床指南，并呼吁采用跨学科方法进行预防和护理。
{"title":"Periodontal medicine: The bidirectional relationship between osteoporosis and periodontal health","authors":"Shivani Sachdeva, H. Saluja, Monica J. Mahajani, Vandana Tripathi, Aparna Deo, Amit Mani","doi":"10.3233/jcb-240150","DOIUrl":"https://doi.org/10.3233/jcb-240150","url":null,"abstract":"Bone resorption is a feature of both osteoporosis and periodontitis. While, periodontitis causes localized inflammatory bone loss after an infected breach of the alveolar cortical bone and may cause tooth loss, osteoporosis causes systemic degenerative bone loss that results in loss of skeletal cancellous microstructure and eventual fracture. The majority of cross-sectional investigations have predominantly used radiographic data and to a lesser extent clinical criteria to confirm the link between osteoporosis and periodontitis. Age, heredity, hormonal changes, smoking, and a lack of calcium and vitamin D are just a few of the many common risk factors. Both disorders may have mutual effects and may even be risk factors for one another, necessitating concurrent therapy. Disruption of the homeostasis of bone remodelling, hormone balance, and inflammation resolution are proposed processes underpinning the relationship. Emerging is a mutual interventional strategy with intricate therapeutic interactions. Both disorders urge future well-controlled longitudinal and interventional studies for evidence-based clinical guidelines and call for interdisciplinary approaches to prevention and care.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140988093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poorna Khaneja, Annette Angel, Vinod Joshi, B. Angel, Shareef Mohammed Buvvaji, S. Mohan, Monika Dheer, Khusbhu Kumari, Ramesh Joshi, Rajesh Thakur, Shilpa Barthwal, A. Khan, N. Peer, Satendar Pal Singh, Neha Singh, Bhawna Sharma, Aarya Chitransh, Reshu Chauhan, Komal Tomar, Kiran Yadav
BACKGROUND: The comparative understanding between cellular basis of transmission and clinical severities caused by Wuhan, Delta and Omicron strains of SARS-CoV-2 is important. OBJECTIVE: To conduct In-Vitro studies on cellular infection caused by different variants by performing cytotoxic assays and studying the live microscopic observations. METHODS: The swab samples positive for Wuhan, Delta and Omicron strains were inoculated in the Vero cell lines and their rate of infectivity and nature of cytopathy was observed. RESULTS: The study showed maximum inter cellular connectivity among cells infected by Delta variant, followed by Wuhan and least inter cellular connections in the cells infected by Omicron variant. The Delta strain causing highest mortality as per reported epidemiological trends, showed highest inter cellular connections and maximum cell damage supporting the fastest cell to cell spread of infection and causing most mortality among patients. CONCLUSIONS: The paper reports that numerous inter cellular connections is the cause of spread of infection among cells and maximum cell damage as the cause of clinical severities. The paper sensitizes the issue that 52 subunit of viral Spike Protein could act as fusion enzyme causing the numerous inter cellular connections. Study suggests that the S2 subunit of the viral Spike Protein could be the drug target for therapeutic intervention.
{"title":"In-vitro studies of relative intracellular alterations caused by three variants of Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV-2)","authors":"Poorna Khaneja, Annette Angel, Vinod Joshi, B. Angel, Shareef Mohammed Buvvaji, S. Mohan, Monika Dheer, Khusbhu Kumari, Ramesh Joshi, Rajesh Thakur, Shilpa Barthwal, A. Khan, N. Peer, Satendar Pal Singh, Neha Singh, Bhawna Sharma, Aarya Chitransh, Reshu Chauhan, Komal Tomar, Kiran Yadav","doi":"10.3233/jcb-240139","DOIUrl":"https://doi.org/10.3233/jcb-240139","url":null,"abstract":"BACKGROUND: The comparative understanding between cellular basis of transmission and clinical severities caused by Wuhan, Delta and Omicron strains of SARS-CoV-2 is important. OBJECTIVE: To conduct In-Vitro studies on cellular infection caused by different variants by performing cytotoxic assays and studying the live microscopic observations. METHODS: The swab samples positive for Wuhan, Delta and Omicron strains were inoculated in the Vero cell lines and their rate of infectivity and nature of cytopathy was observed. RESULTS: The study showed maximum inter cellular connectivity among cells infected by Delta variant, followed by Wuhan and least inter cellular connections in the cells infected by Omicron variant. The Delta strain causing highest mortality as per reported epidemiological trends, showed highest inter cellular connections and maximum cell damage supporting the fastest cell to cell spread of infection and causing most mortality among patients. CONCLUSIONS: The paper reports that numerous inter cellular connections is the cause of spread of infection among cells and maximum cell damage as the cause of clinical severities. The paper sensitizes the issue that 52 subunit of viral Spike Protein could act as fusion enzyme causing the numerous inter cellular connections. Study suggests that the S2 subunit of the viral Spike Protein could be the drug target for therapeutic intervention.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140999555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Spirulina platensis is a well-known photosynthetic prokaryotic alga that has gained recognition in various industries, including food, health, aquaculture and pharmaceuticals. The United Nations acknowledged spirulina as a nutritious food source because of its rich pigments and high protein content. OBJECTIVE: This paper aims to provide an overview of how Spirulina is utilized as both a dietary supplement as well as functional food ingredient in the food industry. METHOD: Spirulina powder can be added to various foods such as bread, cakes and cookies to increase the nutritional value. It is particularly valuable as a dietary supplement in efforts to combat malnutrition in impoverished regions. RESULT: Spirulina platensis is also a source of essential components used in the therapeutic microalgae supplement market. These components include alpha-carotene, astaxanthin, polyunsaturated fatty acids (such as DHA and EPA) and polysaccharides like beta-glucan. CONCLUSION: It is evident that the consumption of spirulina algae can offer both financial benefits and health advantages. Even though there is a large amount of research on the nutritional, environmental and social benefits of spirulina, some natural areas still produce insufficient amounts of the algae. As a result, more and more scientists and researchers throughout the world are pushing for the growth of spirulina farming.
背景： Platensis 螺旋藻是一种著名的光合原核藻类，在食品、保健、水产养殖和制药等各行各业都得到了认可。由于螺旋藻含有丰富的色素和高蛋白，联合国承认螺旋藻是一种营养丰富的食物来源。目的：本文旨在概述螺旋藻在食品工业中如何被用作膳食补充剂和功能性食品配料。方法：螺旋藻粉可以添加到面包、蛋糕和饼干等各种食品中，以增加营养价值。它作为膳食补充剂在消除贫困地区营养不良方面尤其有价值。结果：螺旋藻也是治疗用微藻补充剂市场中使用的重要成分的来源。这些成分包括α-胡萝卜素、虾青素、多不饱和脂肪酸（如 DHA 和 EPA）以及β-葡聚糖等多糖。结论：很明显，食用螺旋藻既能带来经济效益，又能带来健康优势。尽管对螺旋藻的营养、环境和社会效益进行了大量研究，但一些自然地区的螺旋藻产量仍然不足。因此，世界各地越来越多的科学家和研究人员正在推动螺旋藻养殖业的发展。
{"title":"Spirulina platensis: A comprehensive review of its nutritional value, antioxidant activity and functional food potential","authors":"Siramdas Sahil, Suman Bodh, Praveen Verma","doi":"10.3233/jcb-240151","DOIUrl":"https://doi.org/10.3233/jcb-240151","url":null,"abstract":"BACKGROUND: Spirulina platensis is a well-known photosynthetic prokaryotic alga that has gained recognition in various industries, including food, health, aquaculture and pharmaceuticals. The United Nations acknowledged spirulina as a nutritious food source because of its rich pigments and high protein content. OBJECTIVE: This paper aims to provide an overview of how Spirulina is utilized as both a dietary supplement as well as functional food ingredient in the food industry. METHOD: Spirulina powder can be added to various foods such as bread, cakes and cookies to increase the nutritional value. It is particularly valuable as a dietary supplement in efforts to combat malnutrition in impoverished regions. RESULT: Spirulina platensis is also a source of essential components used in the therapeutic microalgae supplement market. These components include alpha-carotene, astaxanthin, polyunsaturated fatty acids (such as DHA and EPA) and polysaccharides like beta-glucan. CONCLUSION: It is evident that the consumption of spirulina algae can offer both financial benefits and health advantages. Even though there is a large amount of research on the nutritional, environmental and social benefits of spirulina, some natural areas still produce insufficient amounts of the algae. As a result, more and more scientists and researchers throughout the world are pushing for the growth of spirulina farming.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Culture inserts of Transwell type are widely used in laboratories worldwide to model non-cerebral vessels, the blood-brain barrier (BBB), and other blood-tissue barriers, and also for the study of chemotaxis and transmigration. However, the use of inserts can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Thus, it is important to develop a method that can reduce the utilization of inserts but not affect research efficiency. In this study, we propose using a 1:1 (v:v) mix of 30% hydrogen peroxide and 99% sulfuric acid (“piranha solution’’) to completely remove cell debris and matrix from culture inserts. BBB models with inserts regenerated using piranha solution have barrier properties comparable to those of fresh inserts. We show that piranha solution is an effective reagent and allows for the reuse of Transwell-type inserts for BBB modeling up to 5 times. Therefore, the use of this method greatly reduces the production of laboratory waste and benefits numerous laboratories worldwide.
{"title":"Regeneration and reuse of transwell-type culture inserts for blood-brain barrier modelling","authors":"Margarita Shuvalova, Georgii Nosov","doi":"10.3233/jcb-240143","DOIUrl":"https://doi.org/10.3233/jcb-240143","url":null,"abstract":"Culture inserts of Transwell type are widely used in laboratories worldwide to model non-cerebral vessels, the blood-brain barrier (BBB), and other blood-tissue barriers, and also for the study of chemotaxis and transmigration. However, the use of inserts can generate plastic waste that has an environmental impact due to their one-off design and massive consumption. Thus, it is important to develop a method that can reduce the utilization of inserts but not affect research efficiency. In this study, we propose using a 1:1 (v:v) mix of 30% hydrogen peroxide and 99% sulfuric acid (“piranha solution’’) to completely remove cell debris and matrix from culture inserts. BBB models with inserts regenerated using piranha solution have barrier properties comparable to those of fresh inserts. We show that piranha solution is an effective reagent and allows for the reuse of Transwell-type inserts for BBB modeling up to 5 times. Therefore, the use of this method greatly reduces the production of laboratory waste and benefits numerous laboratories worldwide.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140376909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nazia Khan, Md Irfanul Haque, Shailesh Shenoy, Rahul Puthenkandathil, Preeti Kale
Molecular dentistry, propelled by advancements in techniques such as Polymerase Chain Reaction (PCR), has emerged as a promising frontier in dental research and practice. PCR has revolutionized the field of dentistry by enabling precise and sensitive detection of various oral pathogens, genetic markers associated with dental diseases, and host response factors. Its ability to amplify specific DNA sequences from minimal starting material has facilitated rapid and accurate diagnosis of dental infections, periodontal diseases, and genetic predispositions to oral conditions. Moreover, PCR-based methods have enhanced our understanding of the oral microbiome composition and its relationship with overall health. This review article aims to elucidate the transformative role of PCR in molecular dentistry by providing an overview of its applications, current developments, and future prospects.
在聚合酶链式反应（PCR）等技术进步的推动下，分子牙科已成为牙科研究和实践中前景广阔的前沿领域。聚合酶链式反应能够精确灵敏地检测各种口腔病原体、与牙科疾病相关的遗传标记以及宿主反应因子，从而彻底改变了牙科领域。它能从最少的起始材料中扩增出特定的 DNA 序列，从而有助于快速准确地诊断牙科感染、牙周疾病和口腔疾病的遗传倾向。此外，基于 PCR 的方法还增进了我们对口腔微生物组组成及其与整体健康关系的了解。这篇综述文章旨在通过概述 PCR 的应用、当前发展和未来前景，阐明 PCR 在分子牙科中的变革性作用。
{"title":"Molecular dentistry: Polymerase chain reaction’s transformative role","authors":"Nazia Khan, Md Irfanul Haque, Shailesh Shenoy, Rahul Puthenkandathil, Preeti Kale","doi":"10.3233/jcb-240147","DOIUrl":"https://doi.org/10.3233/jcb-240147","url":null,"abstract":"Molecular dentistry, propelled by advancements in techniques such as Polymerase Chain Reaction (PCR), has emerged as a promising frontier in dental research and practice. PCR has revolutionized the field of dentistry by enabling precise and sensitive detection of various oral pathogens, genetic markers associated with dental diseases, and host response factors. Its ability to amplify specific DNA sequences from minimal starting material has facilitated rapid and accurate diagnosis of dental infections, periodontal diseases, and genetic predispositions to oral conditions. Moreover, PCR-based methods have enhanced our understanding of the oral microbiome composition and its relationship with overall health. This review article aims to elucidate the transformative role of PCR in molecular dentistry by providing an overview of its applications, current developments, and future prospects.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shivani Sachdeva, Amit Mani, Shweta Vikhe, Anjali Pandit, Pranjali Jagtap
Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. It is one of the most abundant molecule in many of the living organisms due to its connective role in biological structure. It is a highly versatile material, extensively used in the medical, dental, and pharmacological fields. Collagen is capable of being prepared into cross-linked compacted solids or into lattice-like gels. The collagen family consists of at least 30 different genes, which produce 19 known types of collagen. All collagens contain greater or lesser stretches of triple helix. Some of the superior properties of collagen-based biomaterials, such as biocompatibility, biodegradability, mechanical properties, and cell activities, are pinpointed. These properties make collagen applicable in biomedicine, such as wound healing, tissue engineering, surface coating of medical devices, and skin supplementation. The purpose of this article is to review the available literature on collagen with special emphasis on its role in development and maintenance of oral tissues. Also it reviews the types of collagen, it’s structure, sources, role in maintaining periodontal tissues and uses in dentistry.
{"title":"Collagen: A periodontium saviour","authors":"Shivani Sachdeva, Amit Mani, Shweta Vikhe, Anjali Pandit, Pranjali Jagtap","doi":"10.3233/jcb-230136","DOIUrl":"https://doi.org/10.3233/jcb-230136","url":null,"abstract":"Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. It is one of the most abundant molecule in many of the living organisms due to its connective role in biological structure. It is a highly versatile material, extensively used in the medical, dental, and pharmacological fields. Collagen is capable of being prepared into cross-linked compacted solids or into lattice-like gels. The collagen family consists of at least 30 different genes, which produce 19 known types of collagen. All collagens contain greater or lesser stretches of triple helix. Some of the superior properties of collagen-based biomaterials, such as biocompatibility, biodegradability, mechanical properties, and cell activities, are pinpointed. These properties make collagen applicable in biomedicine, such as wound healing, tissue engineering, surface coating of medical devices, and skin supplementation. The purpose of this article is to review the available literature on collagen with special emphasis on its role in development and maintenance of oral tissues. Also it reviews the types of collagen, it’s structure, sources, role in maintaining periodontal tissues and uses in dentistry.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139853412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shivani Sachdeva, Amit Mani, Shweta Vikhe, Anjali Pandit, Pranjali Jagtap
Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. It is one of the most abundant molecule in many of the living organisms due to its connective role in biological structure. It is a highly versatile material, extensively used in the medical, dental, and pharmacological fields. Collagen is capable of being prepared into cross-linked compacted solids or into lattice-like gels. The collagen family consists of at least 30 different genes, which produce 19 known types of collagen. All collagens contain greater or lesser stretches of triple helix. Some of the superior properties of collagen-based biomaterials, such as biocompatibility, biodegradability, mechanical properties, and cell activities, are pinpointed. These properties make collagen applicable in biomedicine, such as wound healing, tissue engineering, surface coating of medical devices, and skin supplementation. The purpose of this article is to review the available literature on collagen with special emphasis on its role in development and maintenance of oral tissues. Also it reviews the types of collagen, it’s structure, sources, role in maintaining periodontal tissues and uses in dentistry.
{"title":"Collagen: A periodontium saviour","authors":"Shivani Sachdeva, Amit Mani, Shweta Vikhe, Anjali Pandit, Pranjali Jagtap","doi":"10.3233/jcb-230136","DOIUrl":"https://doi.org/10.3233/jcb-230136","url":null,"abstract":"Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. It is one of the most abundant molecule in many of the living organisms due to its connective role in biological structure. It is a highly versatile material, extensively used in the medical, dental, and pharmacological fields. Collagen is capable of being prepared into cross-linked compacted solids or into lattice-like gels. The collagen family consists of at least 30 different genes, which produce 19 known types of collagen. All collagens contain greater or lesser stretches of triple helix. Some of the superior properties of collagen-based biomaterials, such as biocompatibility, biodegradability, mechanical properties, and cell activities, are pinpointed. These properties make collagen applicable in biomedicine, such as wound healing, tissue engineering, surface coating of medical devices, and skin supplementation. The purpose of this article is to review the available literature on collagen with special emphasis on its role in development and maintenance of oral tissues. Also it reviews the types of collagen, it’s structure, sources, role in maintaining periodontal tissues and uses in dentistry.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139793269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yugeshwari R. Tiwade, N. Bankar, Vaishnavi Mishra, Anita Sajjanar
Over the past few years, medical artificial intelligence (AI) has been extensively utilized within the healthcare industry. However, the deployment of AI raises complicated social and ethical issues related to security, privacy, and human rights. While the use of artificial intelligence (AI) has the potential to improve healthcare outcomes and operational efficiency, this article gives a detailed assessment of current cutting-edge AI breakthroughs in clinical laboratories. It focuses on the potential benefits of AI and its application in clinical laboratory. The use of AI in clinical laboratory is rapidly growing, with the potential to alter patient care in the near future. Furthermore, it has the potential to democratize modern laboratory services, making them available to people all around the world.
{"title":"Review of the potential benefits and challenges of artificial intelligence in clinical laboratory","authors":"Yugeshwari R. Tiwade, N. Bankar, Vaishnavi Mishra, Anita Sajjanar","doi":"10.3233/jcb-230119","DOIUrl":"https://doi.org/10.3233/jcb-230119","url":null,"abstract":"Over the past few years, medical artificial intelligence (AI) has been extensively utilized within the healthcare industry. However, the deployment of AI raises complicated social and ethical issues related to security, privacy, and human rights. While the use of artificial intelligence (AI) has the potential to improve healthcare outcomes and operational efficiency, this article gives a detailed assessment of current cutting-edge AI breakthroughs in clinical laboratories. It focuses on the potential benefits of AI and its application in clinical laboratory. The use of AI in clinical laboratory is rapidly growing, with the potential to alter patient care in the near future. Furthermore, it has the potential to democratize modern laboratory services, making them available to people all around the world.","PeriodicalId":15286,"journal":{"name":"Journal of Cellular Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139807804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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