热胁迫诱导的小麦肌醇-1-磷酸合成酶2 (TaMIPS2)的过度表达赋予拟南芥对各种非生物胁迫的耐受性

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2017-12-01 DOI:10.1016/j.aggene.2017.09.001

Neetika Khurana, Naveen Sharma, Paramjit Khurana

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引用次数: 9

摘要

肌醇磷酸合酶(MIPS)酶催化肌醇(MI)生物合成的限速步骤。MIPS副产品涉及许多工厂工艺。在这项研究中，从小麦热减法cDNA文库中鉴定的TaMIPS2已被功能表征。为了研究TaMIPS2在耐热性中的重要作用，我们在拟南芥中培养了TaMIPS2过表达基因，并在不同的非生物胁迫条件下对其进行了生理和形态学分析。MIPS酶分析显示了转基因中肌醇的水平，并通过HPLC定量了其他可溶性糖的水平。不同胁迫条件下的过表达基因分析显示TaMIPS2转基因对热胁迫的敏感性降低。这是第一个表明肌醇在热应激中的作用的报告。TaMIPS2转基因株系的分析表明其在其他环境胁迫下具有附加作用。

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Overexpression of a heat stress inducible, wheat myo-inositol-1-phosphate synthase 2 (TaMIPS2) confers tolerance to various abiotic stresses in Arabidopsis thaliana

Myo-Inositol phosphate synthase (MIPS) enzyme catalyses rate limiting step of myo-inositol (MI) biosynthesis. MIPS by-products are involved in many plant processes. In this study, TaMIPS2 identified from a heat subtractive cDNA library from wheat has been functionally characterized. To investigate the importance of TaMIPS2 during heat stress tolerance, TaMIPS2 overexpression transgenics were raised in Arabidopsis which were analysed physiologically and morphologically under different abiotic stress conditions. MIPS enzyme assay revealed the level of inositol in transgenics and of other soluble sugars as quantified by HPLC. Analysis of overexpression transgenics under different stress condition revealed that TaMIPS2 transgenic have reduced sensitivity to heat stress. This is the first report indicating a role of myo-Inositol during heat stress. The analysis of transgenic lines of TaMIPS2 suggests its additional role under other environmental stresses.

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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