不同扦插阶段及添加剂对苏丹高粱青贮发酵品质及微生物群落的影响

IF 3.3 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Fermentation-Basel Pub Date : 2023-08-21 DOI:10.3390/fermentation9080777
Qiang Yu, Mengxin Li, Yu Zhang, Jinyi Xu, Ping Li, Hong Sun, Yixiao Xie, Rui Dong, Yulong Zheng, Chao Chen
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引用次数: 0

摘要

(1) 背景:以往的研究表明,阿魏酸酯酶(FAE)、纤维素酶和木聚糖酶对木质纤维素的降解具有协同作用,切割阶段对青贮饲料的影响较大。这些添加剂是否会影响不同切割阶段的硅烷尚不清楚。(2) 方法:试验扦插阶段的苏丹草高度分别为1.8m(S1)和2.0m(S2)。用产生FAE的植物乳杆菌(LP)、纤维素酶和木聚糖酶(CX)以及LP和CX的组合(LP+CX)处理来自两个切割阶段的青贮饲料30天和60天。(3) 结果:与对照组相比,添加LP+CX可显著降低pH值、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的含量(p<0.05),增加乳酸(LA)浓度(p<0.05)、干物质(DM)含量和粗蛋白含量。添加LP+CX可有效降解苏丹草中的木质纤维素,两个阶段的NDF和ADF降解率均在30%以上。相比之下,S2阶段的切割导致pH较低,LA和DM含量较高(p<0.05)。添加剂和切割阶段对青贮微生物群落产生了强烈的影响,厚壁菌门和乳酸杆菌成为最具优势的细菌门属,尤其是在S2阶段。(4) 结论:FAE生产植物乳杆菌、纤维素酶和木聚糖酶对苏丹草青贮具有协同作用,尤其是在S2阶段,因此它们的使用可以作为一种有效的青贮方法。
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Effects of Different Cutting Stages and Additives on the Fermentation Quality and Microbial Community of Sudangrass (Sorghum sudanense Stapf.) Silages
(1) Background: Previous studies have indicated that ferulic acid esterase (FAE), cellulase and xylanase have synergistic effects in lignocellulose degradation, and the cutting stage has a major impact on silages. Whether these additives affect the silages at different cutting stages is unclear. (2) Methods: Sudangrass height at the tested cutting stages was 1.8 m (S1) and 2.0 m (S2). The silage from the two cutting stages was treated with FAE-producing Lactobacillus plantarum (LP), cellulase and xylanase (CX) and a combination of LP and CX (LP+CX) for 30 and 60 days. (3) Results: Compared with CK, adding LP+CX significantly decreased the pH and the content of neutral detergent fiber (NDF) and acidic detergent fiber (ADF) (p < 0.05) and increased the lactic acid (LA) concentration (p < 0.05), dry matter (DM) content and crude protein content. Adding LP+CX effectively degraded lignocellulose in sudangrass, and the NDF and ADF degradation rates at the two stages were all more than 30%. In comparison, cutting at the S2 stage led to a lower pH and higher LA and DM contents (p < 0.05). Additives and the cutting stage exerted a strong effect on the silage microbial community, and Firmicutes and Lactiplantibacillus became the most dominant bacterial phyla and genera, especially at the S2 stage. (4) Conclusions: The results suggest that FAE-producing L. plantarum, cellulase and xylanase had synergistic effects on sudangrass silages, especially at the S2 stage, and their use can thus serve as an efficient method for ensiling.
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来源期刊
Fermentation-Basel
Fermentation-Basel BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
3.80
自引率
18.90%
发文量
594
审稿时长
7 weeks
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