水稻地方品种半矮化(sd1)基因的标记辅助导入

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2022-03-15 DOI:10.1163/22238980-bja10055
Mankesh Kumar, K. Vaibhav, Anamika Chandra, Kumari Suvidha, Divya Mahto, Satyendra, S. P. Singh, P. K. Singh, S. Sinha
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引用次数: 0

摘要

“卡塔尼大米”是印度比哈尔邦的优质香米品种,具有巨大的出口潜力和商业价值。这是该州的首选大米,因为它的味道、适口性和烹饪前后爆米花般的精华。但近年来,由于其产量低(25-30 Q/公顷),其面积急剧缩小,这是由于其高大、矮小和成熟时的倒伏趋势造成的。为了克服这一问题,尝试了半矮化和高产水稻品种Rajendra Sweta和BPT5204的半矮化(sd1)基因的标记辅助导入。通过109个SSR标记获得亲本多态性。香料和半矮化基因的基因特异性标记被用于分离物中以跟踪它们的转移。对靶基因侧翼的供体基因组片段(即sd1)进行重组选择,然后通过全基因组多态性SSR标记进行背景选择。观察到超过90%的重复亲本基因组恢复,在连续回交世代中,与卡塔尼相比,所选植物的株高和开花时间减少,同时保持了香气。鉴定的品系可以在多位置试验中进一步评估,以将其作为一种可持续的高产芳香基因型进行释放。
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Marker assisted introgression of semi-dwarfing (sd1) gene in Katarni rice (Oryza sativa L.) landrace
‘Katarni Rice’ is a fine quality scented rice landrace of Bihar, India and has tremendous export potential and commercial value. This is a preferred rice in the state due to its flavour, palatability and popcorn like essence before and after cooking. But its area has been shrunken drastically in recent years due to its low yielding (25–30 Q/ha) ability caused due tall and week stature and lodging tendency at maturity. To overcome this problem, marker assisted introgression of semi-dwarfing (sd1) gene from semi-dwarf and high yielding rice varieties Rajendra Sweta and BPT5204 was attempted. Parental polymorphism was obtained through 109 SSR markers. The gene specific markers for fragrance and semi-dwarfing genes were used in the segregants to track their transfer. Recombinant selection was done for the donor genome segments flanking to the target gene i.e., sd1 followed by background selection through genome wide polymorphic SSR markers. More than 90% recurrent parent genome recovery was observed and the selected plants showed a reduction of plant height and flowering time in comparison to Katarni in the successive backcross generation along with the retention of aroma. The identified lines can be evaluated further in multilocation trials to release it as a sustainable high yielding aromatic genotype.
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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