Synthetic seeds technology of Salvia officinalis as a method for short-term storage and in vitro propagation of valuable genotypes

Summary Introduction: The technological advancements in the production of synthetic seeds are critical for the preservation of valuable genotypes of many herbal plants, including Salvia officinalis – sage. Objective: The aim of this study was the production, storage and conversion of artificial sage seeds. The technology of synthetic seeds is placing explants capable of regeneration into plants in a protective casing. Methods: Apical and axillary buds were encapsulated with 1.2% sodium alginate solution, and then dripped in 200 mM CaCl2 solution. Artificial seeds were stored at 4°C for 30 days and then converted on MS medium containing 0.3 mg/l of BAP. Results: The synthetic seeds technology made it possible to obtain a high level of seeds conversion into plants using apical buds (85.0%), and slightly lower in the case of side buds (62.5%). Conclusion: The fully developed technology of synthetic seeds made it possible to obtain a high level of plant viability, which may prove useful for the storage of valuable genotypes of sage.