水芹离体茎尖坏死的控制。

IF 0.9 4区 生物学 Q4 PLANT SCIENCES Israel Journal of Plant Sciences Pub Date : 2020-10-19 DOI:10.1163/22238980-20201046
Hayat Aguinaz, A. Qaddoury, Mohamed Anjarne
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引用次数: 0

摘要

茎尖坏死(STN)是一种生理异常,即顶端茎最初坏死,随后死亡,导致相当大的芽损失,并阻碍角豆(Ceratonia siliqua L.)微繁殖的任何商业应用。本研究的目的是优化在处理一系列培养基化合物的角蛋白体外增殖过程中减轻STN的方法。结果表明,培养基的常量元素强度、细胞分裂素和钙浓度是控制角豆STN发生的最重要因素。事实上,齐默尔曼常量元素在STN减少(仅观察到5%的STN)和芽增殖(28.75芽,26.8叶,4.73厘米长)方面最有效。此外,在Zimmerman或富含Ca的½MS培养基上培养的芽比在低Ca培养基上培育的芽显示出更高的矿物质营养含量。另一方面,从STN中回收的大多数芽在1mg.L-1IAA的存在下产生了根(70%),并且83%在转移到体外条件后存活。Zimmerman宏量元素的性能很可能是由于其与其他介质相比的高Ca浓度(7.3mM)。在富含Ca的½MS上获得的STN强度急剧降低证实了这一点。
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Control of in vitro shoot tip necrosis in Carob Ceratonia siliqua L.
Shoot tip necrosis (STN) is a physiological abnormality whereby the apical shoot initially becomes necrotic and subsequently dies leading to considerable loss of shoots and hampering any commercial application of carob (Ceratonia siliqua L.) micropropagation. The objective of the present study is the optimization of a method to alleviate STN during in vitro multiplication of carob dealing with a range of culture media compounds. Obtained results showed that macroelement strength of the culture medium as well as cytokinin and calcium concentrations were the most important factors in controlling STN incidence in carob. In fact, Zimmerman macroelements are most efficient in terms of STN reduction (only 5% STN observed) and shoot multiplication (28.75 shoots with 26.8 leaves and 4.73 cm length). Moreover, shoots cultured on Zimmerman or Ca enriched ½MS showed higher mineral nutrient contents than those cultured on low Ca media. On the other hand, most shoots recovered from STN have produced roots in presence of 1 mg.L-1 IAA (70%) and 83% survived after transfer to ex vitro conditions. The performance of Zimmerman macroelements is most likely due to its high Ca concentration (7.3 mM) compared to the other media. This is confirmed by the steep reduction of STN intensity obtained on ½MS enriched with Ca.
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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