2011 - 2016年尼日利亚埃努古流行轮状病毒毒株的分子特征

B. Tagbo, Chinedu M. Chukwubike, J. Mwenda, M. Seheri, G. Armah, J. Mphahlele, U. Ozumba, C. Benjamin-Puja, C. Azubuike, H. Okafor, R. Nnani, Vina Okafor, Bo Edelu, C. Eke, O. Udemba, A. Isiaka, L. Namadi, N. Umezinne, R. Njoku, C. Odume, V. Osaro, N. Ogude, M. U. Okwesili, S. K. Ezebilo, K. Yusuf, E. Obidike
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A total of 2694 stool samples were collected from enrolled under 5 years old children with diarrhoea between January 2011 and December 2016 and tested the virus using an antigen enzyme immunoassay. Randomly selected rotavirus positive samples were further characterized by rotavirus genotype methods to identify the G and P types circulating during the study period. Rotavirus was detected in 1242 (46%) of the 2694 samples collected over the six years period. Of these, 867 were randomly selected for genotyping. G and P types could be assigned for 832 samples (96%), while 31 (3.6%) could only be assigned either genotype G or P (partially typed) and 4 (0.4%) could not be assigned genotype G and P (untypeable). The most common G-genotypes detected during the entire study period were G12, G1 and G3 accounting for 27.6%, 21.0% and 16.3% respectively. Mixed G and P-genotypes were commonly detected. 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引用次数: 4

摘要

轮状病毒肠胃炎是全球主要的公共卫生问题,据估计,2013年将导致21.5万名5岁以下儿童死亡;大多数死亡发生在发展中国家。2013年,据估计,尼日利亚是轮状病毒死亡人数第二高的国家。对尼日利亚埃努古循环轮状病毒株的监测是轮状病毒疫苗接种前正在进行的轮状病毒监测的一部分。在2011年1月至2016年12月期间,共从登记的5岁以下腹泻儿童中收集了2694份粪便样本,并使用抗原酶免疫测定法对病毒进行了检测。随机选择的轮状病毒阳性样本通过轮状病毒基因型方法进一步表征,以确定研究期间循环的G型和P型。在六年期间采集的2694份样本中,有1242份(46%)检测到轮状病毒。其中867个是随机选择进行基因分型的。832个样本(96%)可分为G和P型,31个样本(3.6%)只能分为G或P型(部分分型),4个样本(0.4%)不能分为G型和P型(不可分型)。在整个研究期间检测到的最常见的G基因型是G12、G1和G3,分别占27.6%、21.0%和16.3%。G和P混合基因型常见。91个样本(10.8%(91/839)具有混合G基因型,而130个样本(15.2%(130/852))具有混合P基因型。最常见的P基因型为P[8]、P[6]和P[4],分别占38.3%、35.4%和9.1%。检测到的主要毒株是G12P[8](22.3%),其次是G3P[6](14.5%)、G1P[8](9.2%)和G1P[6](8.0%)。这些数据有助于在知情的情况下决定将轮状病毒疫苗纳入国家常规免疫计划,并监测疫苗许可后的影响。
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Molecular Characterization of Rotavirus Strains Circulating in Enugu Nigeria: 2011 to 2016
Rotavirus gastroenteritis is a major public health concern globally, estimated to cause 215,000 deaths among children < 5 years of age in 2013; with majority of mortality occurring in developing countries. In 2013, it was estimated that Nigeria was the second country with the highest number of rotavirus deaths. Monitoring of circulating rotavirus strains in Enugu, Nigeria is part of on-going rotavirus surveillance before the introduction of rotavirus vaccination. A total of 2694 stool samples were collected from enrolled under 5 years old children with diarrhoea between January 2011 and December 2016 and tested the virus using an antigen enzyme immunoassay. Randomly selected rotavirus positive samples were further characterized by rotavirus genotype methods to identify the G and P types circulating during the study period. Rotavirus was detected in 1242 (46%) of the 2694 samples collected over the six years period. Of these, 867 were randomly selected for genotyping. G and P types could be assigned for 832 samples (96%), while 31 (3.6%) could only be assigned either genotype G or P (partially typed) and 4 (0.4%) could not be assigned genotype G and P (untypeable). The most common G-genotypes detected during the entire study period were G12, G1 and G3 accounting for 27.6%, 21.0% and 16.3% respectively. Mixed G and P-genotypes were commonly detected. Ninety-one of the samples, representing 10.8% (91/839) had mixed G-genotype whilst 130 of the samples representing 15.2% (130/852) had mixed P-genotype. The most common P-genotypes detected were P[8], P[6] and P[4] representing 38.3%, 35.4% and 9.1% respectively. The predominant strain detected was G12P[8] (22.3%) followed by G3P[6] (14.5%), G1P[8] (9.2%) and G1P[6] (8.0%). These data are useful for making an informed decision about the introduction of rotavirus vaccine into the national routine immunization program and to monitor the impact of the vaccine post licensure.
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