Production of bioactive compounds by Streptomyces sp. and their antimicrobial potential against selected MDR uropathogens

Multi-drug resistance is increasing day by day and becoming a global health care issue. Hence, there is an urgent need to combat the multidrug resistance uropathogens, so the discovery of novel, efficient, and safe antibiotics from unexplored ecological niches is needed. Actinobacteria have great eminence of their potential for producing bioactive compounds. The aim of the present study is to explore some antimicrobial producing actinobacteria strains isolated from the soil. Our study was carried out with the goal of isolation and characterization of active strain of actinobacteria against uropathogens. Soil samples were collected from the Ganga riverbed, wheat and rice fields, Uttarakhand, India. Actinobacteria were isolated by serial dilution technique. A total of 19 actinobacteria were isolated and cross streaked against multidrug resistant uropathogens. Only seven strains of actinobacteria designed as ACG1, ACG4, ACG5, ACW9, ACW11, ACW14, and ACR17 showed good antimicrobial activity against Pseudomonas aeruginosa, Enterobacter sp., Bacillus subtilis, Staphylococcus aureus uropathogens. Among them, ACG1 showed a broad range of activity against all the tested uropathogens. Furthermore, the extracellular ethyl acetate extract of ACG1 showed maximum zone of inhibition against Enterobacter sp. (23 mm). ACG1 was identified based on morphological, cultural, physiological, and biochemical properties, as well as the 16S ribosomal ribonucleic acid sequence, and was assigned the name S. atacamensis. It was deposited in GenBank under accession number (MW721229). Actinobacteria showed promising results so further studies are required to explore the potential as an antibiotic to cure life-threatening diseases like urinary tract infection.