{"title":"绿色木霉纤维素酶预处理小麦秸秆的糖化动力学","authors":"Milica Galić, M. Stajić, Jasmina Ćilerdžić","doi":"10.35812/cellulosechemtechnol.2022.56.90","DOIUrl":null,"url":null,"abstract":"The main goal of this study was to analyze the potential of Trichoderma viride BEOFB 1210m for cellulase production during solid-state fermentation of wheat straw pretreated with the ligninosome of a well-known white-rot delignificator – Pleurotus pulmonarius HAI 573. After only 7 days of T. viridae BEOFB 1210m cultivation on biologically pretreated wheat straw, this micromycete produced the most active xylanases, which were also the dominant enzymes, with a value of even 3730.10 U L-1. Likewise, maximal but much lower values of exocellulases (155.83 U L-1) and β-glucosidases (59.98 U L-1) were detected after the same period of fermentation. However, much higher activity of endocellulase (2439.55 U L-1) was obtained on the 10th day. The dynamics of enzyme activity was reflected on the level of substrate depolymerization. As much as 30.56% of the cellulose was degraded already on the 7th day, and that percentage did not change significantly until the end of the cultivation period. A significant loss of hemicelluloses was also measured at the beginning of the fermentation process, but it reached a maximum of approximately 50% by the 21st day. The results clearly showed that the selected T. viride strain has very good potential to synthesize highly active cellulases when grown on a cheap and available substrate, which is significant for further large-scale industrial applications.","PeriodicalId":10130,"journal":{"name":"Cellulose Chemistry and Technology","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2022-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DYNAMICS OF PRETREATED WHEAT STRAW SACCHARIFICATION BY CELLULOSOME OF Trichoderma viride\",\"authors\":\"Milica Galić, M. Stajić, Jasmina Ćilerdžić\",\"doi\":\"10.35812/cellulosechemtechnol.2022.56.90\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The main goal of this study was to analyze the potential of Trichoderma viride BEOFB 1210m for cellulase production during solid-state fermentation of wheat straw pretreated with the ligninosome of a well-known white-rot delignificator – Pleurotus pulmonarius HAI 573. After only 7 days of T. viridae BEOFB 1210m cultivation on biologically pretreated wheat straw, this micromycete produced the most active xylanases, which were also the dominant enzymes, with a value of even 3730.10 U L-1. Likewise, maximal but much lower values of exocellulases (155.83 U L-1) and β-glucosidases (59.98 U L-1) were detected after the same period of fermentation. However, much higher activity of endocellulase (2439.55 U L-1) was obtained on the 10th day. The dynamics of enzyme activity was reflected on the level of substrate depolymerization. As much as 30.56% of the cellulose was degraded already on the 7th day, and that percentage did not change significantly until the end of the cultivation period. A significant loss of hemicelluloses was also measured at the beginning of the fermentation process, but it reached a maximum of approximately 50% by the 21st day. The results clearly showed that the selected T. viride strain has very good potential to synthesize highly active cellulases when grown on a cheap and available substrate, which is significant for further large-scale industrial applications.\",\"PeriodicalId\":10130,\"journal\":{\"name\":\"Cellulose Chemistry and Technology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cellulose Chemistry and Technology\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.35812/cellulosechemtechnol.2022.56.90\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, PAPER & WOOD\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellulose Chemistry and Technology","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.35812/cellulosechemtechnol.2022.56.90","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, PAPER & WOOD","Score":null,"Total":0}
引用次数: 0
摘要
本研究的主要目的是分析绿色木霉BEOFB 1210m在用著名白腐脱木素菌——肺菇HAI 573的木质素体预处理的麦草固态发酵过程中产生纤维素酶的潜力。在经过生物预处理的小麦秸秆上仅培养7天的T.viridae BEOFB 1210m后,这种微酵母产生了最具活性的木聚糖酶,木聚糖酶也是主要的酶,甚至达到3730.10U L-1。同样,在相同的发酵期后,检测到胞外纤维素酶(155.83 U L-1)和β-葡萄糖苷酶(59.98 U L-1。然而,在第10天获得了更高的内纤维素酶活性(2439.55U L-1)。酶活性的动态反映在底物解聚的水平上。在第7天,多达30.56%的纤维素已经降解,并且直到培养期结束,这一百分比才发生显著变化。在发酵过程开始时也测量到半纤维素的显著损失,但到第21天达到最大约50%。研究结果清楚地表明,所选的绿色T.viride菌株在廉价可用的底物上生长时,具有合成高活性纤维素酶的良好潜力,这对进一步的大规模工业应用具有重要意义。
DYNAMICS OF PRETREATED WHEAT STRAW SACCHARIFICATION BY CELLULOSOME OF Trichoderma viride
The main goal of this study was to analyze the potential of Trichoderma viride BEOFB 1210m for cellulase production during solid-state fermentation of wheat straw pretreated with the ligninosome of a well-known white-rot delignificator – Pleurotus pulmonarius HAI 573. After only 7 days of T. viridae BEOFB 1210m cultivation on biologically pretreated wheat straw, this micromycete produced the most active xylanases, which were also the dominant enzymes, with a value of even 3730.10 U L-1. Likewise, maximal but much lower values of exocellulases (155.83 U L-1) and β-glucosidases (59.98 U L-1) were detected after the same period of fermentation. However, much higher activity of endocellulase (2439.55 U L-1) was obtained on the 10th day. The dynamics of enzyme activity was reflected on the level of substrate depolymerization. As much as 30.56% of the cellulose was degraded already on the 7th day, and that percentage did not change significantly until the end of the cultivation period. A significant loss of hemicelluloses was also measured at the beginning of the fermentation process, but it reached a maximum of approximately 50% by the 21st day. The results clearly showed that the selected T. viride strain has very good potential to synthesize highly active cellulases when grown on a cheap and available substrate, which is significant for further large-scale industrial applications.
期刊介绍:
Cellulose Chemistry and Technology covers the study and exploitation of the industrial applications of carbohydrate polymers in areas such as food, textiles, paper, wood, adhesives, pharmaceuticals, oil field applications and industrial chemistry.
Topics include:
• studies of structure and properties
• biological and industrial development
• analytical methods
• chemical and microbiological modifications
• interactions with other materials