Detection of compound antibody of anti-HI with wide thermal amplitude in plasma of a pregnant woman

Objective To investigate the identification and characteristics of anti-HI compound antibody with wide thermal amplitude. Methods In March 2017, one pregnant woman with positive irregular antibody who identified irregular antibody specificity in Blood Group Research Department of Dalian Blood Center was selected as the object of this study. The pregnant woman was 27 years old, with AB, RhD positive blood group, and 37 weeks pregnant. In the past, this pregnant woman had a surviving child through caesarean section, and no history of blood transfusion. A volume of 5 mL peripheral blood was collected from pregnant women and her husband for blood group identification, direct anti-human globulin test, and screening and identification of irregular antibodies. Saline tube method was used for blood group identification. Direct anti-human globulin test used tube method. Screening and identification of immunoglobulin (Ig) M irregular antibodies used saline test tube method. This study was in line with World Medical Association Declaration of Helsinki revised in 2013 and informed contents were obtained from the subjects. Results ① The forward grouping of the pregnant women was A1B, the reverse grouping results were negative for A cells, weak agglutination for B cells and 2+ agglutination for O cells. The results of forward and reserve grouping of ABO blood group were not conformed, and irregular antibodies were present. ② The results of direct anti-human globulin test in this pregnant woman showed that both anti-IgG and anti-C3d were negative. ③ The results of antibody screening test showed there was IgM anti-HI compound antibody with wide thermal amplitude in her plasma. Conclusions For patients carried anti-IH compound antibody with wide thermal amplitade, their compound antibody should be screened and identificated by various methods and reagents. And avoid transfusion of ABO mismatched red blood cells for these patients. Key words: Antibodies; Anti-HI; Compound antibody; Transfusion; Antibody screening; Pan-agglutination