V. Campos, R. Kaspary, M. Rossi, T. A. M. Medeiros
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引用次数: 3
摘要
将地衣芽孢杆菌接种在不同底物上,通过发酵生产γ-聚谷氨酸。在深层发酵(SmF)中,甘油和葡萄糖作为主要的碳底物,硫酸铵提供氮。在固态发酵(SSF)中,以大豆为主要底物。在含有地衣芽孢杆菌的气升式生物反应器中进行的测试显示,最大生产力为0.789 g L–1 h–1,产量为0.4 g g–1。2015年和2016年作物的不同大豆品种被用于SSF。来自2016年作物的BRS 1001IPRO品种表现出最高的产量,即1.2 g L–1的γ-PGA。对该品种进行不同程度的碾磨(细、中、粗)。粗磨谷物使γ-PGA产量增加到2.7 g L–1。此外,粗磨使谷物的内部营养物质可用于发酵,并且这种颗粒大小不会影响发酵过程中的通气。
Biosynthesis of γ-Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF)
Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L–1 h–1 and a yield of 0.4 g g–1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L–1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L–1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.
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