口服尼古丁对6-羟多巴胺诱导双侧腹外侧纹状体损伤的雄性大鼠的细胞、行为和运动的影响

IF 0.3 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Clinical and Experimental Health Sciences Pub Date : 2023-07-05 DOI:10.33808/clinexphealthsci.1162013
Elif SARICA DAROL, Ayşe Karson, S. Köktürk, Pervin İşeri̇
{"title":"口服尼古丁对6-羟多巴胺诱导双侧腹外侧纹状体损伤的雄性大鼠的细胞、行为和运动的影响","authors":"Elif SARICA DAROL, Ayşe Karson, S. Köktürk, Pervin İşeri̇","doi":"10.33808/clinexphealthsci.1162013","DOIUrl":null,"url":null,"abstract":"Objective: Parkinson's disease is a progressive neurodegenerative disease having a spectrum of non-motor to motor symptoms. Unrelated to motor symptoms of sensory, autonomic, and neuropsychiatric symptoms often appear early in the course of the disease. It is a remarkable observation that patients in the premotor phase can easily quit smoking without help. This study was intended to investigate the interrelation between nicotine and the partial loss of dopaminergic innervation in the ventrolateral striatum induced by 6-OHDA. \nMethods: We used an experimental premotor parkinsonism model. The oral nicotine preference of rats was investigated with the two-bottle free choice method. The behaviors related to locomotor activity and emotional state were evaluated with a locomotor activity test, elevated plus maze, and forced swimming test. Histopathological evaluation was performed in the striatum by staining techniques using hematoxylin+eosin (H&E) and immunohistochemistry markers (caspase-3, and MAP-2). \nResults: Bilateral 6-OHDA lesions did not lead to a significant alteration in the total locomotor activity or nicotine preference. Nicotine increased horizontal but decreased vertical movements in addition to increasing anxiolytic but also depressive effects in the OHDA lesion group. The number of apoptotic cells was significantly lower in the lesion group receiving nicotine compared to those not receiving nicotine. \nConclusion: Our experimental study points to the role of oral nicotine in male rats with bilateral striatal 6-OHDA lesions in the ventrolateral striatum. Further studies are needed to understand the relationship between loss of dopaminergic innervation in the striatum and nicotine consumption.","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cellular, Behavioral, and Locomotor Effects of Oral Nicotine in Male Rats with Bilateral Lesions in the Ventrolateral Striatum Induced with 6-OHDA\",\"authors\":\"Elif SARICA DAROL, Ayşe Karson, S. Köktürk, Pervin İşeri̇\",\"doi\":\"10.33808/clinexphealthsci.1162013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Parkinson's disease is a progressive neurodegenerative disease having a spectrum of non-motor to motor symptoms. Unrelated to motor symptoms of sensory, autonomic, and neuropsychiatric symptoms often appear early in the course of the disease. It is a remarkable observation that patients in the premotor phase can easily quit smoking without help. This study was intended to investigate the interrelation between nicotine and the partial loss of dopaminergic innervation in the ventrolateral striatum induced by 6-OHDA. \\nMethods: We used an experimental premotor parkinsonism model. The oral nicotine preference of rats was investigated with the two-bottle free choice method. The behaviors related to locomotor activity and emotional state were evaluated with a locomotor activity test, elevated plus maze, and forced swimming test. Histopathological evaluation was performed in the striatum by staining techniques using hematoxylin+eosin (H&E) and immunohistochemistry markers (caspase-3, and MAP-2). \\nResults: Bilateral 6-OHDA lesions did not lead to a significant alteration in the total locomotor activity or nicotine preference. Nicotine increased horizontal but decreased vertical movements in addition to increasing anxiolytic but also depressive effects in the OHDA lesion group. The number of apoptotic cells was significantly lower in the lesion group receiving nicotine compared to those not receiving nicotine. \\nConclusion: Our experimental study points to the role of oral nicotine in male rats with bilateral striatal 6-OHDA lesions in the ventrolateral striatum. Further studies are needed to understand the relationship between loss of dopaminergic innervation in the striatum and nicotine consumption.\",\"PeriodicalId\":10192,\"journal\":{\"name\":\"Clinical and Experimental Health Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2023-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Health Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33808/clinexphealthsci.1162013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Health Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33808/clinexphealthsci.1162013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

摘要

目的:帕金森病是一种进行性神经退行性疾病,具有一系列非运动到运动症状。与运动症状无关的感觉、自主神经和神经精神症状常出现在病程早期。运动前期的患者在没有帮助的情况下很容易戒烟,这是一个值得注意的观察结果。本研究旨在探讨尼古丁与6-羟多巴胺引起的腹外侧纹状体多巴胺神经支配部分丧失的关系。方法:采用实验性运动前帕金森病模型。采用两瓶自由选择法研究大鼠对口服尼古丁的偏好。采用运动活动测试、高架+迷宫测试和强迫游泳测试评估运动活动和情绪状态的相关行为。采用苏木精+伊红(H&E)染色技术和免疫组织化学标记(caspase-3和MAP-2)对纹状体进行组织病理学评估。结果:双侧6-OHDA损伤未导致总运动活动或尼古丁偏好的显著改变。尼古丁增加了水平运动,但减少了垂直运动,此外还增加了OHDA病变组的抗焦虑和抑郁作用。与未接受尼古丁治疗的损伤组相比,接受尼古丁治疗的损伤组的凋亡细胞数量明显减少。结论:本实验研究指出了口服尼古丁对双侧侧纹状体6-OHDA损伤的雄性大鼠的作用。纹状体多巴胺能神经支配的丧失与尼古丁消耗之间的关系需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Cellular, Behavioral, and Locomotor Effects of Oral Nicotine in Male Rats with Bilateral Lesions in the Ventrolateral Striatum Induced with 6-OHDA
Objective: Parkinson's disease is a progressive neurodegenerative disease having a spectrum of non-motor to motor symptoms. Unrelated to motor symptoms of sensory, autonomic, and neuropsychiatric symptoms often appear early in the course of the disease. It is a remarkable observation that patients in the premotor phase can easily quit smoking without help. This study was intended to investigate the interrelation between nicotine and the partial loss of dopaminergic innervation in the ventrolateral striatum induced by 6-OHDA. Methods: We used an experimental premotor parkinsonism model. The oral nicotine preference of rats was investigated with the two-bottle free choice method. The behaviors related to locomotor activity and emotional state were evaluated with a locomotor activity test, elevated plus maze, and forced swimming test. Histopathological evaluation was performed in the striatum by staining techniques using hematoxylin+eosin (H&E) and immunohistochemistry markers (caspase-3, and MAP-2). Results: Bilateral 6-OHDA lesions did not lead to a significant alteration in the total locomotor activity or nicotine preference. Nicotine increased horizontal but decreased vertical movements in addition to increasing anxiolytic but also depressive effects in the OHDA lesion group. The number of apoptotic cells was significantly lower in the lesion group receiving nicotine compared to those not receiving nicotine. Conclusion: Our experimental study points to the role of oral nicotine in male rats with bilateral striatal 6-OHDA lesions in the ventrolateral striatum. Further studies are needed to understand the relationship between loss of dopaminergic innervation in the striatum and nicotine consumption.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Clinical and Experimental Health Sciences
Clinical and Experimental Health Sciences MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
0.00%
发文量
73
期刊最新文献
Presumptive Molecular Interconnections Between COVID-19 And Huntington’s Disease Enhanced Enzyme Inhibitory Effects of the Nanohybrid Eggplant Extract: An Unusual Pharmaceutical Form for Medicinal Plant The Validity and Reliability of the Postpartum Symptom Inventory in Turkish Women The Effect of Sex Education and Counseling Based on the Ex-PLISSIT Model on Sexual Life in Primigravidas Ketogenic Diet Interventions in Inborn Errors of Metabolism: A Review Article
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1