巴基斯坦化妆品中细菌的分离与检测

Q4 Agricultural and Biological Sciences Proceedings of the Pakistan Academy of Sciences: Part B Pub Date : 2023-01-19 DOI:10.53560/ppasb(60-sp1)815
Tazeen, Sidra Rahman, Rizwan Abbas, Syed Ahsan Shahid, Z. Shinwari, Muhammad Ishtiaq Ali
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引用次数: 0

摘要

化妆品是我们日常生活中最基本、最常用的成分。除了改善人类健康,它们还提供健康的生活方式,增强我们的自尊。预计2021年全球化妆品市场将达到2870亿美元,2022年将达到4150亿美元。本研究旨在分离、鉴定和鉴定化妆品中的细菌菌株。将不同的化妆品接种在胰蛋白酶大豆琼脂培养基上,分离出6个菌落。所有菌株在37°C下均表现出最佳生长。使用不同的培养基,如MacConkey琼脂、SIM和Simmons柠檬酸盐琼脂,通过生化测试评估所有菌株,并通过Sanger测序进一步进行核苷酸测序。在化妆品中发现了不同的菌株,包括少动鞘氨醇单胞菌、海洋芽孢杆菌、Robertmurraya andreesnii、坚定细胞杆菌、苍白Falsbacillus pallidus和杜松不动杆菌。这些菌株中的大多数被发现是致病性的,然而,鞘氨醇单胞菌具有生物修复的潜力,可以用来降解有毒化合物,使环境变得更好。类似地,细胞杆菌被发现参与生物矿化,也有助于发酵。我们的研究结果表明,迫切需要确保严格的化妆品安全法规。不当的生产做法可能导致化妆品污染,从而导致健康质量恶化的严重后果。需要进一步的研究来探索这些分离物的潜力,以便利用它们来改善我们的健康和环境。
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Isolation and Detection of Bacterial Strains from Cosmetics Products Available in Pakistan
Cosmetics products are the most essential and frequently used components in our daily life. Besides improving human health, they provide healthy lifestyles and boost our self-esteem. Globally cosmetics market is projected to be 287 billion USD in 2021 to 415 billion USD in 2022. This research study aims at the isolation, identification, and characterization of bacterial strains isolated from cosmetics. Six bacterial colonies were isolated by inoculating different cosmetics products on tryptic soya agar media. All the strains showed optimum growth at 37 °C. All strains were assessed through biochemical tests by using different media such as MacConkey agar, SIM, and Simmons citrate agar and were further proceeded for nucleotide sequencing through Sanger sequencing. Different bacterial strains were revealed in cosmetics products including Sphingomonas paucimobilis, Cytobacillus oceanisediminis, Robertmurraya andreesnii, Cytobacillus firmus, Falsibacillus pallidus, and Acinetobacter junii. Most of these strains were found to be pathogenic however, Sphingomonas has the potential for bioremediation and can be utilized for degrading toxic compounds to make the environment better. Similarly, Cytobacillus is found to be involved in biomineralization and also aids in fermentation. Our results have shown that there is a dire need to assure strict safety regulations regarding cosmetics. Improper manufacturing practices can lead to the contamination of cosmetics which could lead to severe consequences of deteriorating the quality of health. Further studies are needed to explore the potential of these isolates so that they can be utilized to improve our health as well as the environment.
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来源期刊
Proceedings of the Pakistan Academy of Sciences: Part B
Proceedings of the Pakistan Academy of Sciences: Part B Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
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