谷氨酸棒状杆菌合成生物学技术及工业应用研究进展

IF 3.3 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Fermentation-Basel Pub Date : 2023-08-04 DOI:10.3390/fermentation9080729
Yujue Wang, Qiang Wang, Aobo Sha, Kexin Ren, Mengkai Hu, Meijuan Xu, Xian Zhang, Zhigang Rao
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引用次数: 0

摘要

谷氨酸棒状杆菌是一种革兰氏阳性细菌(不形成孢子),已被广泛用于氨基酸生产。谷氨酰胺因其蛋白分泌稳定、胞外水解酶活性低、无毒等优点,极大地拓展了其应用领域。目前,基于合成生物学的基因编辑技术由于能够有效调控谷氨酰胺的生理代谢网络,在谷氨酰胺的合成生物学研究和基因改造方面具有很大的潜力。因此,我们从基因修饰和表达元件方面对谷氨酰胺的基因编辑工具和策略进行了总结,并描述了基因编辑技术对氨基酸及其衍生物、重组蛋白、功能糖等多种产物的影响,为谷氨酰胺菌株的修饰研究和工业应用提供一定的理论依据。最后,基于基因编辑技术,从多个角度对谷氨酰胺基因改造的设计和产业化应用进行了展望。
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Advances in Synthetic Biology Techniques and Industrial Applications of Corynebacterium glutamicum
Corynebacterium glutamicum is a Gram-positive bacterium (non-spore-forming) that has been wildly used for amino acid production. Due to its stable protein secretion, low extracellular hydrolase activity, and non-toxicity, the application field of C. glutamicum has been greatly expanded. Currently, gene editing technology based on synthetic biology has great potential for synthetic biology research and genetic modification in C. glutamicum because of its ability to efficiently regulate the physiological and metabolic networks of the strain. Therefore, we summarize the gene editing tools and strategies of C. glutamicum from the aspects of genetic modification and expression elements, and we also describe the effects of gene editing techniques on a variety of products such as amino acids and their derivatives, recombinant proteins, and functional sugars, which provide a certain theoretical basis for the research on the modification of C. glutamicum strains and industrial applications. Finally, we prospect the design and industrial application of C. glutamicum genetic modification from multiple perspectives based on gene editing techniques.
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来源期刊
Fermentation-Basel
Fermentation-Basel BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
3.80
自引率
18.90%
发文量
594
审稿时长
7 weeks
期刊最新文献
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