湘西牛子宫和卵巢差异表达基因的转录组学分析

IF 0.7 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY Pakistan Journal of Agricultural Sciences Pub Date : 2022-10-01 DOI:10.21162/pakjas/21.657
Wu Song, Jianbo Li, Ao Sun, Y. Long
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引用次数: 0

摘要

小母牛的繁殖效率直接取决于生殖系统的健康,尤其是卵巢和子宫的健康。本实验采用RNA测序(RNAseq)和基因集富集分析(GSEA)技术,筛选与生殖性状相关的候选基因,检测DEG的分子机制,了解环境适应性、分子育种和生殖性能分析。此外,本实验还利用Illumina II高通量测序技术对湘西牛子宫和卵巢之间的转录组进行了测序,并对不同基因的通路进行了分析。将组装的卵巢总数与参考基因组序列进行比较,进行DEGs分析、GO和KEGG富集分析以及候选基因筛选。基于转录组数据,利用总RNA构建了7个cDNA文库。在七个签约的cDNA文库中,在每个测序文库中,原始读数达到>133Mb,并且保留了>94%的干净读数。将湘西牛卵巢与子宫进行比较,共发现3635个mRNA,其中1608个表达显著上调,2027个表达下调。ADCY3、ADCY4、ADCY9、FSHR、BMP15、LHCGR、INSR、CYP11A1、CYP17A1、CYP19A1、STAR和SCARB1在湘西牛卵巢和子宫组织中的mRNA表达存在显著差异。通过GSEA和PPI网络,我们发现卵巢类固醇生成途径在湘西牛卵巢组织发育过程中起着重要的信号调节作用
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Transcriptomic analysis of differentially expressed genes between uterus and ovary in Xiangxi cattle
Reproductive efficiency of a heifer is directly dependent on the health of reproductive system especially ovary and uterus. Current experiment was carried out to screen candidate genes related to reproductive traits and detect molecular mechanisms of DEGs by using RNA-sequencing (RNAseq) and Gene Set Enrichment Analysis (GSEA) to understand the environmental adaptability, molecular breeding and analyze the reproductive performance. Furthermore, Illumina II high throughput sequncing technology was utilized in the current experiment to sequence transcriptomes and analysis related to pathway of different genes between uterus and ovary in Xiangxi cattle. The total number of ovary assembled was compared to the reference genome sequence, DEGs analysis, GO and KEGG enrichment analysis and candidate gene screening were performed. Based on the transcriptome data, seven cDNA libraries were constructed by utilizing total RNA. Out of seven contracted cDNA libraries, in every sequencing library, raw reads reached >133 Mb, and >94% clean reads remained. After comparing the Xiangxi cattle ovaries with the uterus, a total of 3635 mRNAs were found, in which 1608 were significantly up-regulated expressed and 2027 were down-regulated expression mRNAs. The mRNA expression of ADCY3, ADCY4, ADCY9, FSHR, BMP15, LHCGR, INSR, CYP11A1, CYP17A1, CYP19A1, STAR and SCARB1 in ovary of Xiangxi Cattle were significant different in ovarian and uterus tissues. Through GSEA and PPI network, we found the ovarian steroidogenesis pathway played significant role in signal regulation during the development of the ovarian tissue of the Xiangxi cattle
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来源期刊
Pakistan Journal of Agricultural Sciences
Pakistan Journal of Agricultural Sciences AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.80
自引率
25.00%
发文量
18
审稿时长
6-12 weeks
期刊介绍: Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.
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