Pham Ha Thanh Tung, Tran Van on, Phung Thanh Huong
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Assessment of Genetic Variation of the Genus Gymnema in Vietnam, Using RAPD and ITS-rDNA Markers
ABSTRACT Gymnema is a genus of the Apocynaceae with more than 50 species. Some of its species such as G. sylvestre and G. latifolium have been reported for their medicinal value. In this study, the genetic diversity of 20 samples of four species of Gymnema in Vietnam was evaluated using the Random Amplification of Polymorphic DNA (RAPD) and the internal transcribed spacer of nuclear ribosomal DNA (ITS-rDNA). Amplified bands obtained with primers UBC701, UBC709, and OPC4 were the highest polymorphic in RAPD analysis. The phylogenetic trees were built grouping the 20 samples into four clusters corresponding to the four Gymnema species according to morphological classification. A number of characteristic SNPs were identified for each species which can be used for authentication and differentiation. The results showed the consensus and usefulness of the two barcoding techniques in the molecular identification and characterization of the Gymnema genus.
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The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.
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