用埃文斯蓝染料测定啮齿动物血脑屏障完整性

Q2 Immunology and Microbiology Current Protocols in Immunology Pub Date : 2019-07-19 DOI:10.1002/cpim.83

Mariana Pereira de Souza Goldim, Amanda Della Giustina, Fabricia Petronilho

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引用次数: 73

摘要

血脑屏障(BBB)是一种主动和选择性屏障，保护大脑免受内源性和外源性损伤。不同的刺激可能导致这一屏障的破坏，包括炎症和创伤。几种方法用于评估血脑屏障的破坏。应用最广泛的方法是埃文斯蓝(EB)染料外渗。EB通常不能通过血脑屏障，因此它在脑组织中的存在表明其渗透性的改变。本方案详细说明了EB在啮齿动物体内外渗的步骤。还提供了有关关键步骤和优势的重要方面。©2019 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Using Evans Blue Dye to Determine Blood-Brain Barrier Integrity in Rodents

The blood-brain barrier (BBB) is an active and selective barrier that shields the brain from endogenous and exogenous insults. Different stimuli may lead to the disruption of this barrier, including inflammation and trauma. Several methods are used to evaluate BBB disruption. The most widely used method is Evans blue (EB) dye extravasation. EB cannot normally pass through the BBB and thus its presence in brain tissue indicates alterations in permeability. This protocol details the steps of EB extravasation in rodents. Important aspects regarding critical steps and advantages are also provided. © 2019 by John Wiley & Sons, Inc.

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Current Protocols in Immunology Immunology and Microbiology-Immunology

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