杨柳多酚耐药转运蛋白的全基因组鉴定及对重金属胁迫的表达分析

IF 3.3 2区 农林科学 Q1 AGRONOMY Agronomy-Basel Pub Date : 2023-09-06 DOI:10.3390/agronomy13092330
Shuxin Chen, Juanjuan Chen, Zhuchou Lu, Yuhan Jia, Yuying Yang, R. Zhuo, Xiaojiao Han
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引用次数: 0

摘要

多效耐药(PDR)转运体是atp结合盒转运体ABCG亚家族的一部分,已被发现参与重金属耐受。柳属植物(柳树)生物量大、耐受性强、重金属积累能力强,被广泛认为是植物修复重金属的理想候选者。然而,柳树中PDR家族对重金属反应的系统发育和机制尚未确定。在本研究中,我们发现并评估了紫荆柳基因组中共21个PDR基因。利用这些基因的系统发育关系将它们划分为5个主要分支。SpPDRs在不同分支上的外显子-内含子分布模式和基因长度存在差异。在pdr启动子中发现了与胁迫反应、干旱诱导、低温和防御反应相关的顺式作用元件。重金属胁迫下,不同组织中PDR基因转录水平存在显著差异,不同重金属调控不同PDR成员。在根中,PDR4和PDR21表现出高表达水平。同时,PDR7和PDR17在所有被分析的组织中显示出相似的转录模式。此外,PDR5和PDR16之间存在显著的正相关,而PDR3和PDR9之间存在显著的负相关,这表明PDR成员对重金属的反应是复杂和多方面的。这些发现将为理解PDR基因的生物学功能,特别是它们参与柳树对重金属耐受性的调控奠定重要基础。
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Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in Salix purpurea and Expression Analysis in Response to Various Heavy Metal Stresses
Pleiotropic drug resistance (PDR) transporters, which are part of the ABCG subfamily of ATP-binding cassette (ABC) transporters, have been found to be involved in heavy metal tolerance. Salix species (willow) is widely regarded as a perfect candidate for phytoremediation of heavy metals because of its substantial biomass, strong tolerance, and remarkable capacity to accumulate heavy metals. However, the phylogeny and mechanisms underlying the response to heavy metals within the PDR family in willow have yet to be determined. In this study, we discovered and valuated a total of 21 PDR genes in the genome of Salix purpurea. The phylogenetic relationships of these genes were used to classify them into five major clades. The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow’s tolerance to heavy metals.
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来源期刊
Agronomy-Basel
Agronomy-Basel Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍: Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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