Shuxin Chen, Juanjuan Chen, Zhuchou Lu, Yuhan Jia, Yuying Yang, R. Zhuo, Xiaojiao Han
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The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow’s tolerance to heavy metals.","PeriodicalId":56066,"journal":{"name":"Agronomy-Basel","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in Salix purpurea and Expression Analysis in Response to Various Heavy Metal Stresses\",\"authors\":\"Shuxin Chen, Juanjuan Chen, Zhuchou Lu, Yuhan Jia, Yuying Yang, R. 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Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in Salix purpurea and Expression Analysis in Response to Various Heavy Metal Stresses
Pleiotropic drug resistance (PDR) transporters, which are part of the ABCG subfamily of ATP-binding cassette (ABC) transporters, have been found to be involved in heavy metal tolerance. Salix species (willow) is widely regarded as a perfect candidate for phytoremediation of heavy metals because of its substantial biomass, strong tolerance, and remarkable capacity to accumulate heavy metals. However, the phylogeny and mechanisms underlying the response to heavy metals within the PDR family in willow have yet to be determined. In this study, we discovered and valuated a total of 21 PDR genes in the genome of Salix purpurea. The phylogenetic relationships of these genes were used to classify them into five major clades. The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow’s tolerance to heavy metals.
Agronomy-BaselAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍:
Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.