解淀粉芽孢杆菌U17产脲酶和碳酸酐酶菌株CaCO3的沉淀及性质

IF 1.1 4区 生物学 Q3 BIOLOGY Turkish Journal of Biology Pub Date : 2019-06-13 DOI:10.3906/biy-1901-56
M. Tepe, Ş. Arslan, T. Koralay, N. Mercan Doğan
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引用次数: 12

摘要

本研究分析了从Denizli(土耳其)石灰性土壤中分离的解淀粉芽孢杆菌U17的碳酸钙矿化特性以及脲酶和碳酸酐酶活性。CaCO3在所有生长阶段都产生。菌株U17在钙矿化培养基中表现出0.615±0.092µmol/min/mg脲酶酶活性,在添加尿素的Luria Bertani培养基中显示出1.315±0.021µmol/min/g脲酶酶活性,而在CaCO3沉淀培养基中显示出36.03±5.48nmol/min/mg碳酸酐酶活性,在添加尿素的Luria Bertani培养基中表现出28.82±3.31nmol/min/mg碳酸酐酶活性。首次用蛋白质印迹法检测菌株U17尿素酶B蛋白的表达水平。通过X射线衍射、X射线荧光、共聚焦拉曼光谱、扫描电子显微镜和电子探针微量分析仪对制备的CaCO3晶体进行分析,以评估其形态和元素性质。通过矿物学分析,可以清楚地检测和验证菱形球霰石和层状方解石晶体。所有这些结果表明,U17菌株由于其CaCO3沉淀能力,可以在许多工程和地质应用中使用。
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Precipitation and characterization of CaCO3 of Bacillus amyloliquefaciens U17 strain producing urease and carbonic anhydrase
In the present study, the properties of calcium carbonate mineralization and urease and carbonic anhydrase activities of Bacillus amyloliquefaciens U17 isolated from calcareous soil of Denizli (Turkey) were analyzed. CaCO3 was produced in all growth phases. Strain U17 showed 0.615 ± 0.092 µmol/min/mg urease enzyme activity in calcium mineralization medium and 1.315 ± 0.021 µmol/min/mg urease enzyme activity in Luria-Bertani medium supplemented with urea, whereas it showed 36.03 ± 5.48 nmol/min/mg carbonic anhydrase enzyme activity in CaCO3 precipitation medium and 28.82 ± 3.31 nmol/min/mg carbonic anhydrase enzyme activity in Luria-Bertani medium supplemented with urea. The urease B protein expression level of strain U17 was detected by western blotting for the first time. The produced CaCO3 crystals were analyzed by X-ray diffraction, X-ray fluorescence, confocal RAMAN spectrophotometer, scanning electron microscopy, and electron probe microanalyzer for the evaluation of their morphological and elemental properties. Rhombohedral vaterite and layered calcite crystals were clearly detected and verified by mineralogical analyses. All these results showed that strain U17 can be used in many engineering and geological applications due to its CaCO3 precipitation ability.
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.
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