编码调节脂肪酸代谢和细胞对脂质反应的蛋白质的基因在长期培养的猪腔上皮中差异表达

Q4 Biochemistry, Genetics and Molecular Biology Medical Journal of Cell Biology Pub Date : 2019-10-12 DOI:10.2478/acb-2019-0008
M. Kulus, B. Borowiec, M. Popis, P. Celichowski, M. Ješeta, D. Bukowska, H. Piotrowska-Kempisty, M. Bruska, M. Zabel, M. Nowicki, B. Kempisty, P. Antosik
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引用次数: 3

摘要

摘要在许多因素中,输卵管管腔上皮对卵母细胞的发育及其随后的受精非常重要。输卵管上皮的特征是存在纤毛细胞,支持卵丘-卵母细胞复合体向子宫移动。通过与精液的相互作用,输卵管上皮使精子获得受精的能力。到目前为止,这些变化的确切分子机制尚不清楚。因此,了解输卵管上皮细胞的代谢和单个基因组的表达水平似乎是加深对广泛理解的生殖知识的一种方式。在我们的研究中,我们决定在体外长期培养输卵管上皮细胞。在24、7、15和30天后,收获OECs,分离其RNA。使用微阵列分析转录组的变化。“细胞对脂质的反应”组由以下基因表示:MUC1、CYP24A1、KLF4、IL24、SNAI2、CXCL10、PPARD、TNC、ABCA10,而属于“细胞脂质代谢过程”的基因为:LIPG、ARSK、ACADL、FADS3、P2RX7、ACSS2、PPARD和KITLG、SPTLC3、ERBB3、KLF4和CRABP2。此外,PPARD和ACADL是“脂肪酸β氧化”本体论小组的成员。我们的研究描述了与生育过程没有直接关系的基因。然而,它们在体外培养的OECs中表达的显著变化可能表明它们作为OECs生理过程的标记物是有用的。标题:猪输卵管上皮细胞体外培养过程中脂肪酸的变化
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Genes encoding proteins regulating fatty acid metabolism and cellular response to lipids are differentially expressed in porcine luminal epithelium during long-term culture
Abstract Among many factors, the epithelium lining the oviductal lumenis very important for the development of the oocyte and its subsequent fertilization. The oviductal epithelium is characterized by the presence of ciliary cells, supporting the movement of cumulus-oocyte complexes towards the uterus. By interacting with the semen, the epithelium of the fallopian tube makes the sperm acquire the ability to fertilize. So far, the exact molecular mechanisms of these changes have not been known. Hence, understanding the metabolism of oviduct epithelial cells and the level of expression of individual groups of genes seems to be a way to deepen the knowledge about the broadly understood reproduction. In our research, we decided to culture oviductal epithelial cells (OECs) in vitro for a long period of time. After 24h, 7, 15 and 30 days, the OECs were harvested, with their RNA isolated. Transcriptomic changes were analyzed using microarrays. The “cellular response to lipid” group was represented by the following genes: MUC1, CYP24A1, KLF4, IL24, SNAI2, CXCL10, PPARD, TNC, ABCA10, while the genes belonging to the “cellular lipid metabolic processes” were: LIPG, ARSK, ACADL, FADS3, P2RX7, ACSS2, PPARD, KITLG, SPTLC3, ERBB3, KLF4, CRABP2. Additionally, PPARD and ACADL were members of the “fatty acid beta-oxidation” ontology group. Our study describes genes that are not directly related to fertility processes. However, significant changes in their expression in in vitro cultured OECs may indicate their usefulness as markers of OECs’ physiological processes. Running title: Fatty acids changes in porcine oviductal epithelial cells in in vitro cultivation
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Medical Journal of Cell Biology
Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
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