Effect of Plant Growth Regulators on in Vitro Propagation of Paris Polyphylla

ABSTRACT In vitro propagation using plant growth regulators (PGRs) was evaluated in Paris polyphylla. Thin cell layers (0.5–1 mm) of rhizomes (explants) were sliced and inoculated on half-strength MS (Murashige and Skoog) medium with 3% (w/v) sucrose and solidified with 0.3% (w/v) clerigel. In this medium, 50% of the explants were treated with (0.5 µg mL−1) activated charcoal (AC) and 50% without AC. The half strength MS medium was supplemented with different concentrations of 6-benzylaminopurine (BAP) either individually or in combinations with auxins. The optimal percentage (80%) of shoot response was induced by the synergistic action of BAP (0.5 µg mL−1) and α-naphthalene acetic acid (NAA) (0.2 µg mL−1) in the medium containing AC. After sub-culturing, the highest number (4.00 ± 1.00) of shoot buds per explant was achieved in the MS medium with AC, which was augmented with a combination of BAP (0.5 µg mL−1) and NAA (0.2 µg mL−1). The medium containing AC showed greater shoot induction than the non-AC medium. Up to 94.4% of the regenerated shoots successfully established in the greenhouse soil.