水解多糖单加氧酶10家族生物降解辅助活性的异源表达研究进展

IF 3.3 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Fermentation-Basel Pub Date : 2023-08-28 DOI:10.3390/fermentation9090795
Hongyu Zhang, Zixuan Zhou, Tingting Lou, Rong Xiang, Deguang Zhang, Danyun Wang, Suying Wang
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引用次数: 0

摘要

AA10家族裂解多糖单加氧酶(AA10 LPMOs)主要分布在细菌中。由于其氧化降解纤维素和几丁质等结晶多糖的特性,在工业生物质转化方面具有很大的应用潜力,受到了广泛的关注。利用重组工程菌高效异源表达LPMOs已成为酶工业生产的主要策略。本文综述了AA10 LPMOs异源表达系统的研究进展。从表达宿主、载体和LPMOs基因的设计与加工等方面介绍了其多样化异源表达体系的构建策略。讨论了不同表达体系对LPMOs可溶性表达的影响,以及构建LPMOs异源表达体系的发展方向。展望了LPMOs在生物质转化和生物燃料工业中的广阔应用前景。
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Advance in Heterologous Expression of Biomass-Degrading Auxiliary Activity 10 Family of Lytic Polysaccharide Monooxygenases
AA10 family lytic polysaccharide monooxygenases (AA10 LPMOs) are mainly distributed in bacteria. Because of their characteristics of oxidative degradation of crystalline polysaccharides, such as cellulose and chitin, they have great application potential in industrial biomass conversion and have attracted wide attention. Efficient heterologous expression of LPMOs by recombinant engineering bacteria has become the main strategy for the industrial production of enzymes. The research progress of AA10 LPMOs’ heterologous expression systems was reviewed in this paper. The construction strategies of its diversified heterologous expression system were introduced based on the design and processing of the expression host, vector, and LPMOs gene. The effects of different expression systems on the soluble expression of LPMOs and the development direction of the construction of LPMOs’ heterologous expression systems were discussed. The broad application prospect of LPMOs in the biomass conversion and biofuel industry has been prospected.
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来源期刊
Fermentation-Basel
Fermentation-Basel BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
3.80
自引率
18.90%
发文量
594
审稿时长
7 weeks
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