{"title":"烟叶半边莲不同叶、根提取物自由基清除活性评价及EC50值估算(野生烟草)","authors":"R. Kolap, Prachi S. Kakade, R. Gacche, S. Zimare","doi":"10.1080/10496475.2021.1932006","DOIUrl":null,"url":null,"abstract":"ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.","PeriodicalId":35803,"journal":{"name":"Journal of Herbs, Spices and Medicinal Plants","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10496475.2021.1932006","citationCount":"3","resultStr":"{\"title\":\"Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco)\",\"authors\":\"R. Kolap, Prachi S. Kakade, R. Gacche, S. Zimare\",\"doi\":\"10.1080/10496475.2021.1932006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.\",\"PeriodicalId\":35803,\"journal\":{\"name\":\"Journal of Herbs, Spices and Medicinal Plants\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2021-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10496475.2021.1932006\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbs, Spices and Medicinal Plants\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10496475.2021.1932006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbs, Spices and Medicinal Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10496475.2021.1932006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco)
ABSTRACT The antioxidant potential (% RSA and EC50) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC50 values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL−1) and hydrogen peroxide (81.01 µg mL−1) radical assays. The % RSA and EC50 correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.
期刊介绍：
The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.
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