{"title":"响应面法优化金萱夏绿茶中表没食子儿茶素没食子酸酯的提取工艺","authors":"Xiang Limin, Shunshun Pan, Xingfei Lai, Lingli Sun, Zhigang Li, Qiuhua Li, Ya-hui Huang, Sun Shili","doi":"10.5073/JABFQ.2018.091.022","DOIUrl":null,"url":null,"abstract":"The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2018-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Optimization of brewing conditions in epigallocatechin-3-gallate (EGCG) extraction from Jinxuan summer green tea by response surface methodology\",\"authors\":\"Xiang Limin, Shunshun Pan, Xingfei Lai, Lingli Sun, Zhigang Li, Qiuhua Li, Ya-hui Huang, Sun Shili\",\"doi\":\"10.5073/JABFQ.2018.091.022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.\",\"PeriodicalId\":56276,\"journal\":{\"name\":\"Journal of Applied Botany and Food Quality-Angewandte Botanik\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2018-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Botany and Food Quality-Angewandte Botanik\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.5073/JABFQ.2018.091.022\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Botany and Food Quality-Angewandte Botanik","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.5073/JABFQ.2018.091.022","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Optimization of brewing conditions in epigallocatechin-3-gallate (EGCG) extraction from Jinxuan summer green tea by response surface methodology
The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.
期刊介绍：
The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.
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