多肽TK14功能化疏水涂层提高生物医用镁合金的耐腐蚀性和生物相容性

IF 1.6 Q4 ENGINEERING, BIOMEDICAL Biosurface and Biotribology Pub Date : 2021-06-06 DOI:10.1049/bsb2.12011
Yang Shen, Xiaolong Shen, Hao Zhang, Xin Li, Tengda Shang, Yuancong Zhao, Jin Wang, Nan Huang
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引用次数: 1

摘要

国家自然科学基金项目,批准号:32071328,81801853;四川省科技计划项目,批准号:19GJHZ0058;国家重点研发计划,批准号:2017YFB 0702500摘要镁及其合金具有优异的力学性能和生物相容性,可作为生物医学材料。然而,镁基材料的快速降解速率限制了其在可生物降解血管内支架中的应用。为了克服这个问题,我们在镁上构建了一种疏水涂层。在用碱和纯镁硅烷偶联剂预处理后,4,4'-二苯基甲烷二异氰酸酯(MDI)和氨基封端的聚二甲基硅氧烷(H2N–PDMS–NH2)逐步沉积在表面,形成含氨基疏水涂层(–(M/P)3)以提高耐腐蚀性。此外,多肽TK14被固定在疏水涂层上,以促进血管内皮细胞的粘附和增殖。电化学结果显示,与纯Mg相比,–(M/P)3的自腐蚀电流密度(icorr)降低了约4.5个数量级。TK14固定化后,粘附在–(M/P)3–T表面的内皮细胞数量显著增加。尽管–(M/P)3–T的耐腐蚀性略有降低,但周围组织的皮下植入炎症反应较低,显示出合适的生物相容性。因此,多肽TK14功能化疏水涂层可能是镁基心血管植入物界面的一种有前途的候选材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Improved corrosion resistance and biocompatibility of biomedical magnesium alloy with polypeptide TK14 functionalised hydrophobic coating

Magnesium (Mg) and its alloys can be used as biomedical materials because of their excellent mechanical properties and biocompatibility. However, the rapid degradation rate of Mg-based materials limits their application in biodegradable intravascular stents. To overcome this issue, we constructed a hydrophobic coating on magnesium. After pre-treatments with alkali and a silane coupling agent of pure magnesium, 4,4’-diphenylmethane-diisocyanate (MDI) and amino-terminated polydimethylsiloxane (H2N–PDMS–NH2) were stepwise deposited on the surface, forming an amino-containing hydrophobic coating (–(M/P)3) to enhance the corrosion resistance. Furthermore, polypeptide TK14 was immobilised on the hydrophobic coating to promote vascular endothelial cell adhesion and proliferation. The electrochemical results revealed that the self-corrosion current density (icorr) of –(M/P)3 decreased by approximately 4.5 orders of magnitude compared with that of pure Mg. After TK14 immobilisation, the number of endothelial cells adhering to the surface of –(M/P)3–T increased significantly. Although the corrosion resistance of –(M/P)3–T was slightly reduced, the subcutaneous implantation inflammatory response of the surrounding tissues was lower, showing suitable biocompatibility. Therefore, the polypeptide TK14 functionalised hydrophobic coating may be a promising candidate material for the interface of magnesium-based cardiovascular implants.

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来源期刊
Biosurface and Biotribology
Biosurface and Biotribology Engineering-Mechanical Engineering
CiteScore
1.70
自引率
0.00%
发文量
27
审稿时长
11 weeks
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