{"title":"预测温度对色谱分离性能影响的简化方法","authors":"S. Nozaki, N. Yoshimoto, Shuichi Yamamoto","doi":"10.11301/jsfe.21591","DOIUrl":null,"url":null,"abstract":"A simplified method for predicting the effect of temperature on the separation performance by chromatography was developed. As a model separation system, two polyphenol component separation by polymer-resin chromatography with ethanol-water mobile phase was chosen. HETP values as a function of mobile phase velocity for a wide range of temperatures 15-45 ℃ were well correlated by a single universal curve in the dimensionless HETP and the dimensionless velocity plots. A simplified equation that can predict the resolution was derived. The equation includes the temperature dependence of the distribution coefficients and the molecular diffusion coefficients. The relative separation performance (resolution) calculated by this equation showed that the existence of the maximum resolution depends on the temperature dependence of the distribution coefficients. Even when the resolution decreases with temperature, higher temperature operations may be advantageous as both the separation time and the separation volume decrease.","PeriodicalId":39399,"journal":{"name":"Japan Journal of Food Engineering","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Simplified Method for Predicting the Effect of Temperature on the Separation Performance by Chromatography\",\"authors\":\"S. Nozaki, N. Yoshimoto, Shuichi Yamamoto\",\"doi\":\"10.11301/jsfe.21591\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A simplified method for predicting the effect of temperature on the separation performance by chromatography was developed. As a model separation system, two polyphenol component separation by polymer-resin chromatography with ethanol-water mobile phase was chosen. HETP values as a function of mobile phase velocity for a wide range of temperatures 15-45 ℃ were well correlated by a single universal curve in the dimensionless HETP and the dimensionless velocity plots. A simplified equation that can predict the resolution was derived. The equation includes the temperature dependence of the distribution coefficients and the molecular diffusion coefficients. The relative separation performance (resolution) calculated by this equation showed that the existence of the maximum resolution depends on the temperature dependence of the distribution coefficients. Even when the resolution decreases with temperature, higher temperature operations may be advantageous as both the separation time and the separation volume decrease.\",\"PeriodicalId\":39399,\"journal\":{\"name\":\"Japan Journal of Food Engineering\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japan Journal of Food Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11301/jsfe.21591\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Engineering\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japan Journal of Food Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11301/jsfe.21591","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Engineering","Score":null,"Total":0}
A Simplified Method for Predicting the Effect of Temperature on the Separation Performance by Chromatography
A simplified method for predicting the effect of temperature on the separation performance by chromatography was developed. As a model separation system, two polyphenol component separation by polymer-resin chromatography with ethanol-water mobile phase was chosen. HETP values as a function of mobile phase velocity for a wide range of temperatures 15-45 ℃ were well correlated by a single universal curve in the dimensionless HETP and the dimensionless velocity plots. A simplified equation that can predict the resolution was derived. The equation includes the temperature dependence of the distribution coefficients and the molecular diffusion coefficients. The relative separation performance (resolution) calculated by this equation showed that the existence of the maximum resolution depends on the temperature dependence of the distribution coefficients. Even when the resolution decreases with temperature, higher temperature operations may be advantageous as both the separation time and the separation volume decrease.
期刊介绍：
The Japan Society for Food Engineering (the Society) publishes "Japan Journal of Food Engineering (the Journal)" to convey and disseminate information regarding food engineering and related areas to all members of the Society as an important part of its activities. The Journal is published with an aim of gaining wide recognition as a periodical pertaining to food engineering and related areas.
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