基于乙酰胆碱酯酶活性抑制的加惹河水质评价

F. Riswanto, M. Hariono, Gatot Nugroho Susanto, Ignatius Yulius Kristio Budiasmoro, E. Istyastono
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引用次数: 1

摘要

日惹的一条河流ASBTRAKSungai Gajah Wong担心,由于生活垃圾的填埋、工业、医院或酒店进入河流,会出现水污染。这会导致河流水质下降,并对使用河流的生物产生负面影响。根据有机磷和氨基甲酸酯类农药污染对乙酰胆碱酯酶(AChE)的抑制活性测试,可以对河流的水污染进行生化监测。水质分析中针对AChE抑制的生物化学测试可以根据Ellman方法以色度法进行。这种反应可以通过有色人种社会的形成来观察,其制服可以用可见波的长度来测量。在考虑污染负荷/扩散污染负荷的情况下,确定研究中的采样位置。设置了6个河流采样点,从北到南依次为GW-1、GW-2、GW-3、GW-4、GW-5和GW-6。从第六个位置开始,作为采样线中最南端的GW-6显示出最高的AChE活性抑制,表现为低AChE活力。日惹市的河流之一ASBTRACTGajahWong河因将家庭、工业、医院和酒店的废物排入河流而受到水污染物污染的威胁。这些污染可能会导致水质下降,并对开发利用水质的生物产生负面影响。通过有机磷和氨基甲酸酯类农药污染对乙酰胆碱酯酶(AChE)活性的抑制,可以从生化角度观察到河流污染。水质分析中的生化测定可以根据埃尔曼法的比色法进行。该反应的结果可以通过在可见波长下测量有色化合物的形成来观察。本研究中采样位置的确定是基于扩散污染负荷的考虑。定义并命名了六个采样点,从北到南依次为GW-1、GW2、GW3、GW4、GW-5和GW-6。结果表明,作为采样点中最南端的GW-6的水对AChE活性的抑制作用最高。
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KUALITAS AIR SUNGAI GAJAH WONG DITINJAU DARI PENGHAMBATAN ENZIM ASETILKOLINESTERASE (Water Quality Assessment of Gajah Wong River Based on Inhibition of Acetylcholinesterase Activity)
ASBTRAKSungai Gajah Wong, salah satu sungai di kota Yogyakarta, dikhawatirkan terpapar pencemaran air akibat pembuangan limbah rumah tangga, industri, rumah sakit maupun hotel yang masuk ke dalam alirannya. Hal ini menyebabkan penurunan kualitas air sungai dan menimbulkan dampak negatif bagi makhluk hidup yang memanfaatkannya. Pencemaran air sungai tersebut dapat diamati secara biokimiawi berdasarkan uji aktivitas penghambatan enzim asetilkolinesterase (AChE) oleh cemaran pestisida organofosfat dan karbamat. Uji biokimiawi dalam analisis kualitas air terhadap penghambatan AChE dapat dilakukan secara kolorimetrik menurut metode Ellman. Hasil reaksi ini dapat diamati secara melalui pembentukan senyawa berwarna dan serapannya dapat diukur pada panjang gelombang visibel. Penentuan lokasi sampling pada penelitian didasarkan pada pertimbangan sebaran muatan polusi/diffuse pollution loads consideration. Ditetapkan enam lokasi pengambilan sampel air sungai yaitu GW-1, GW-2, GW-3, GW-4, GW-5, dan GW-6 berturut-turut dari utara ke selatan. Dari keenam lokasi, GW-6 sebagai titik paling selatan dalam jalur pengambilan sampel menunjukkan penghambatan aktivitas AChE yang paling tinggi ditunjukkan dengan aktivitas AChE yang rendah. ASBTRACTGajah Wong River, one of the rivers in Yogyakarta city, was threatened by contamination of water pollutant due to the disposal waste of households, industrials, hospitals and hotels into its stream. The pollutions might cause the decreasing of water quality and raised the negative impact to living creatures that exploit it. River pollutions can be observed biochemically based on inhibition of acetylcholinesterase (AChE) enzyme activity by organophosphate and carbamate pesticide contamination. Biochemical assay in the analysis of water quality could be done according to the colorimetric of Ellman’s method. The results of this reaction can be observed by measuring the formation of colored compounds at visible wavelengths. Determination of the sampling location in this research was performed based on diffuse pollution loads consideration. Six sampling sites was defined and named as GW-1, GW 2, GW 3, GW 4, GW-5, and GW-6, respectively in a row from north to south. As the results, water from GW-6 as the southernmost site in the sampling sites showed the highest inhibition of AChE activity. 
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