粘土聚合物纳米复合材料介导的蛋白质聚集抑制:在预防蛋白质病中的可能作用。

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein and Peptide Letters Pub Date : 2023-10-04 DOI:10.2174/0109298665274059231002071951
Romana Parveen, Sher Ali, Sadaf Fatima
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引用次数: 0

摘要

背景:蛋白质在特定条件下由于错误折叠和聚集而从天然构象转变为高度有序的原纤维结构,被描述为富含β片的淀粉样蛋白原纤维。这些原纤维在身体不同部位的积聚是几种神经和非神经疾病(蛋白质病)的主要原因。目的:为了预防这些蛋白质病,抑制蛋白质聚集被认为是一种很有前途的策略。因此,在本研究中,我们合成了基于蒙脱石(MMT)的聚邻苯二胺(PoPD)纳米复合材料(NCs),并对其尺寸和形态进行了表征。此外,评估了这些纳米复合材料对原纤维形成的抑制作用。方法:采用硫黄素T(ThT)和1-苯胺基-8-萘磺酸盐(ANS)荧光、刚果红染料结合分析(CR)等生物物理方法,评价了这些纳米复合材料对淀粉样变性的两种模型蛋白,即人溶菌酶和人血清白蛋白(HL&HSA)的抗淀粉样变性潜力。二级结构含量通过圆二色性(CD)光谱进行评估。结果:结果表明,合成的纳米复合材料以剂量依赖的方式显著抑制原纤维的形成,这与它们阻止纤颤的能力相对应。有人认为,当它们受到聚集条件时,它们可以吸附蛋白质以保护它们免受聚集。结论:本研究为了解纳米复合材料抑制原纤维形成的机制提供了机会,表明它们可以抑制淀粉样蛋白的形成和淀粉样蛋白疾病。因此,该研究得出结论,这些纳米复合材料是蛋白质疾病治疗分子的有前途的候选者,有望丰富个性化医学领域,增强人类医疗保健系统。
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Clay-Polymer Nanocomposites Mediated Inhibition of Protein Aggregation: Possible Role in the Prevention of Proteinopathies.

Background: The transformation of proteins from their native conformation into highly ordered fibrillar structures due to their misfolding and aggregation under particular conditions are described as beta-sheet enriched amyloid fibrils. The accumulation of these fibrils in different body parts is the major cause of several neurological and non-neurological conditions (proteinopathies).

Objectives: To prevent these proteinopathies, inhibition of protein aggregation is considered a promising strategy. Therefore, in this study, we synthesized montmorillonite (MMT) based poly- orthophenylenediamine (PoPD) nanocomposites (NCs) and characterized their size and morphology due to their remarkable biological properties. Further, the effect of these nanocomposites on inhibition of fibril formation was assessed.

Methods: These nanocomposites were evaluated for their anti-amyloidogenic potential on two model proteins of amyloidopathies, i.e., human lysozyme and human serum albumin (HL & HSA), by using several biophysical methods, such as Thioflavin T (ThT) and 1-anilino-8-naphthalene sulfonate (ANS) fluorescence, congo red dye binding assay (CR). Secondary structural content was evaluated by Circular dichroism (CD) spectroscopy.

Results: Results demonstrated that synthesized nanocomposites significantly inhibited fibril formation in dose-dependent manner that corresponds to their ability to arrest fibrillation. It is suggested that they may adsorb proteins to protect them against aggregation when they are subjected to aggregating conditions.

Conclusion: This study offers an opportunity to understand the mechanism of inhibition of fibril formation by nanocomposites, showing that they inhibit amyloid formation and amyloid diseases. Thus, the study concludes that these nanocomposites are promising candidates as therapeutic molecules for proteinopathies and are envisaged to enrich the area of personalized medicine, augmenting the human healthcare system.

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来源期刊
Protein and Peptide Letters
Protein and Peptide Letters 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
98
审稿时长
2 months
期刊介绍: Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis
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