用于香料掺假检测的灵敏和方便用户的等位基因特异性PCR检测方法的开发

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2023-09-18 DOI:10.1016/j.jarmap.2023.100518
Xu Zhang, Ruiying Wang, Dandan Wang, Hongtao Wang
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引用次数: 0

摘要

香料掺假不仅严重干扰其调味功能,还会导致消费者中毒危及生命。为了克服传统方法在香料掺假检测中的局限性,开发了一种多重等位基因特异性PCR系统,用于对四种常用香料——小茴香进行分子鉴别。,花椒。,八角和香茅&;L.M.Perry,来自基于叶绿体SNP标记的相应掺杂物。所开发的检测方法消除了DNA测序和假阴性结果的障碍,可以检测0.1%至0.01ng水平的香料掺假基因组DNA,具有绝对的等位基因特异性和良好的效率。在此基础上,建立了利用多重等位基因特异性PCR检测香料掺假的标准操作程序。因此,本研究为香料掺假检测提供了一种简单、可靠、灵敏的分子方法。
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Development of a sensitive and user-friendly allele-specific PCR assay for adulteration detection of spices

Spice adulteration not only seriously interferes with their flavoring functions but also leads to life-threatening poisoning for consumers. To overcome the limitations of traditional methods in spice adulteration detection, a multiplex allele-specific PCR system was developed for molecular discrimination of four commonly used spices, Foeniculum vulgare Mill., Zanthoxylum bungeanum Maxim., Illicium verum Hook.f., and Syzygium aromaticum (L.) Merr. & L.M.Perry, from their corresponding adulterants based on chloroplast SNP markers. The developed assay, eliminating the obstacles of DNA sequencing and false negative results, can detect 0.1% of spice adulteration down to 0.01 ng level of genomic DNA with absolute allelic specificity and favorable efficiency. Based on the results, a standard operating procedure for using multiplex allele-specific PCR for spice adulteration detection was established. Therefore, the present study provided a simple, reliable, and sensitive molecular method for adulteration detection of spices.

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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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