小麦千粒重稳定QTL的精细定位与验证

IF 6 1区 农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-10-01 DOI:10.1016/j.cj.2023.03.007
Deyuan Meng , Aamana Batool , Yazhou Xuan , Ruiqing Pan , Na Zhang , Wei Zhang , Liya Zhi , Xiaoli Ren , Wenqing Li , Jijie Li , Yanxiao Niu , Shuzhi Zheng , Jun Ji , Xiaoli Shi , Lei Wang , Hongqing Ling , Chunhua Zhao , Fa Cui , Xigang Liu , Junming Li , Liqiang Song
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引用次数: 0

摘要

千粒重(TKW)是衡量小麦育种目标粒重的指标。本研究的目的是在克农9204(KN9204)和京411(J411)杂交的重组自交系(RIL)群体中精细定位TKW的稳定数量性状位点(QTL),并鉴定其候选基因。在高密度遗传连锁图上,24、26和25个QTL分别与TKW、粒长(KL)和粒宽(KW)相关。一个主要且稳定的QTL,QTkw-2D,被定位到染色体臂2DL上8.3cM的区间。通过靶区多态性标记的饱和,QTkw-2D被限制在9.13Mb的物理区间内,使用来自残余杂合子系的二次定位群体(F6:7)。使用QTkw-2D近等基因系(NILs)将该间隔进一步缩小到2.52Mb。在不同灌浆阶段,NILsKN9204的鲜重和干重均高于NILsJ411。在田间试验中,NILsKN9204的TKW和KW远高于NILsJ411。通过比较KN9204和J411的DNA序列和表达,将TraesCS2D02G460300.1(TraesKN2D01HG49350)确定为QTkw-2D的候选基因。通过QTkw-2D NIL的RNA测序(RNA-seq)证实了这一点。这些结果为基于图谱的QTkw-2D克隆提供了基础,与候选基因连接的DNA标记可用于标记辅助选择。
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Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.)

Thousand-kernel weight (TKW) is a measure of grain weight, a target of wheat breeding. The object of this study was to fine-map a stable quantitative trait loci (QTL) for TKW and identify its candidate gene in a recombinant inbred line (RIL) population derived from the cross of Kenong 9204 (KN9204) and Jing 411 (J411). On a high-density genetic linkage map, 24, 26 and 25 QTL were associated with TKW, kernel length (KL), and kernel width (KW), respectively. A major and stable QTL, QTkw-2D, was mapped to an 8.3 cM interval on chromosome arm 2DL. By saturation of polymorphic markers in its target region, QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line (F6:7). This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines (NILs). NILsKN9204 had higher fresh and dry weights than NILsJ411 at various grain-filling stages. The TKW and KW of NILsKN9204 were much higher than those of NILsJ411 in field trials. By comparison of both DNA sequence and expression between KN9204 and J411, TraesCS2D02G460300.1 (TraesKN2D01HG49350) was assigned as a candidate gene for QTkw-2D. This was confirmed by RNA sequencing (RNA-seq) of QTkw-2D NILs. These results provide the basis of map-based cloning of QTkw-2D, and DNA markers linked to the candidate gene may be used in marker-assisted selection.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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