叶片损伤MIMIC 4的破坏影响水稻ABA合成和ROS积累

IF 6 1区 农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-10-01 DOI:10.1016/j.cj.2023.04.001
Hao Wu , Gaoxing Dai , Rao Yuchun , Kaixiong Wu , Junge Wang , Peng Hu , Yi Wen , Yueying Wang , Lixin Zhu , Bingze Chai , Jialong Liu , Guofu Deng , Qian Qian , Jiang Hu
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引用次数: 0

摘要

病变模拟突变体是研究程序性细胞死亡(PCD)的有利材料。虽然一些水稻LMM基因已被克隆,但这些基因功能的多样性表明,LMM中细胞死亡调控的机制有待进一步研究。在这项研究中,我们鉴定了一个水稻光依赖性叶片病变模拟突变体4 (llm4),该突变体表现出叶绿体结构异常、光抑制、光合蛋白水平降低、活性氧(ROS)大量积累和PCD。基于图谱的克隆和互补测试表明,LLM4编码玉米黄素环氧酶(ZEP), ZEP是一种参与叶黄素循环的酶,在植物光保护、ROS清除和类胡萝卜素和脱落酸(ABA)的生物合成中起作用。ABA含量降低,24种类胡萝卜素含量与野生型(WT)存在差异。与WT相比,llm4突变体表现出更少的休眠,对ABA更敏感。我们得出结论,llm4突变导致叶黄素循环失败,进而引起ROS积累。过量的ROS积累破坏叶绿体结构,诱发PCD,最终导致病变模拟物的形成。
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Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice

Lesion mimic mutants (LMMs) are advantageous materials for studying programmed cell death (PCD). Although some rice LMM genes have been cloned, the diversity of functions of these genes indicates that the mechanism of cell death regulation in LMMs needs further study. In this study, we identified a rice light-dependent leaf lesion mimic mutant 4 (llm4) that showed abnormal chloroplast structure, photoinhibition, reduced photosynthetic protein levels, massive accumulation of reactive oxygen species (ROS), and PCD. Map-based cloning and complementation testing revealed that LLM4 encodes zeaxanthin epoxidase (ZEP), an enzyme involved in the xanthophyll cycle, which functions in plant photoprotection, ROS scavenging, and carotenoid and abscisic acid (ABA) biosynthesis. The ABA content was decreased, and the contents of 24 carotenoids differed between the llm4 mutant and the wild type (WT). The llm4 mutant showed reduced dormancy and greater sensitive to ABA than the WT. We concluded that the mutation of LLM4 resulted in the failure of xanthophyll cycle, in turn causing ROS accumulation. The excessive ROS accumulation damaged chloroplast structure and induced PCD, leading eventually to the formation of lesion mimics.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
期刊最新文献
Editorial Board Increasing Fusarium verticillioides resistance in maize by genomics-assisted breeding: Methods, progress, and prospects Serotonin enrichment of rice endosperm by metabolic engineering GmTOC1b negatively regulates resistance to Soybean mosaic virus Ectopic expression of OsNF-YA8, an endosperm-specific nuclear factor Y transcription-factor gene, causes vegetative and reproductive development defects in rice
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