Jia-Xing Fang, Da-Feng Chen, Xia Shi, Su-Fang Zhang, Fu Liu, Wei-Xing Shen, Chun-Yan Jia, Shen-Cheng Ma, Zhen Zhang, Xiang-Bo Kong
{"title":"固相微萃取和表皮碳氢化合物差异与刺柏二孔虫繁殖活性的关系","authors":"Jia-Xing Fang, Da-Feng Chen, Xia Shi, Su-Fang Zhang, Fu Liu, Wei-Xing Shen, Chun-Yan Jia, Shen-Cheng Ma, Zhen Zhang, Xiang-Bo Kong","doi":"10.1111/jse.12947","DOIUrl":null,"url":null,"abstract":"<p>Cuticular hydrocarbons of Cerambycidae species can function as signals for sex recognition. Little is known about the copulatory signals of the juniper bark borer <i>Semanotus bifasciatus</i>, a major economic threat to <i>Platycladus orientalis</i> Franco in China. Here, we investigated the cuticular hydrocarbons of both sexes of <i>S. bifasciatus</i> to determine the chemically mediated mating signals using the solid-phase microextraction (SPME) technique with carbowax/divinylbenzene fibers (CAR/DVB) and then analyzed by coupled gas chromatography-mass spectrometry (GC-MS). A series of aliphatic saturated straight-chain <i>n</i>-alkanes (<i>n</i>-C<sub>23</sub> to <i>n</i>-C<sub>28</sub>), internally branched monomethylalkanes at carbons 3, 11, or 13, and dimethylalkanes were identified, which showed no qualitative differences in either sex and were similar in the samples with SPME fiber extraction and those with hexane extraction. The bioassay showed that 11-methylpentacosane (11-MeC<sub>25</sub>), 11-methylhexacosane (11-MeC<sub>26</sub>), and 11-methylheptacosane (11-MeC<sub>27</sub>) have sex-specific recognition functions that triggered more mating attempts at a female-specific ratio of 100:4:60 than at a male-specific ratio of 100:85:50. In addition, the female-specific ratio of 11-methylalkanes can elicit about 70% of male mating attempts within about 60 s, whereas live females elicit about 98% of male mating attempts within 25 s. The discrepancy in the initiation of mating attempts by synthetic mixtures and live females suggests that the methyl isomers 3-MeC<sub>25</sub>, 3-MeC<sub>27</sub>, and/or 11,15-diMeC<sub>27</sub> may also be involved in the mating behavior of <i>S. bifasciatus</i>. These results suggest that 11-MeC<sub>25</sub>, 11-MeC<sub>26</sub>, and 11-MeC<sub>27</sub> constitute the contact sex pheromone of <i>S. bifasciatus</i>, with the presence or absence of 11-MeC<sub>26</sub> in particular playing an important role in mate recognition by males.</p>","PeriodicalId":17087,"journal":{"name":"Journal of Systematics and Evolution","volume":"61 3","pages":"498-505"},"PeriodicalIF":3.7000,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jse.12947","citationCount":"1","resultStr":"{\"title\":\"Solid-phase microextraction and cuticular hydrocarbon differences related to reproductive activity in juniper bark borer Semanotus bifasciatus Motschulsky\",\"authors\":\"Jia-Xing Fang, Da-Feng Chen, Xia Shi, Su-Fang Zhang, Fu Liu, Wei-Xing Shen, Chun-Yan Jia, Shen-Cheng Ma, Zhen Zhang, Xiang-Bo Kong\",\"doi\":\"10.1111/jse.12947\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Cuticular hydrocarbons of Cerambycidae species can function as signals for sex recognition. Little is known about the copulatory signals of the juniper bark borer <i>Semanotus bifasciatus</i>, a major economic threat to <i>Platycladus orientalis</i> Franco in China. Here, we investigated the cuticular hydrocarbons of both sexes of <i>S. bifasciatus</i> to determine the chemically mediated mating signals using the solid-phase microextraction (SPME) technique with carbowax/divinylbenzene fibers (CAR/DVB) and then analyzed by coupled gas chromatography-mass spectrometry (GC-MS). A series of aliphatic saturated straight-chain <i>n</i>-alkanes (<i>n</i>-C<sub>23</sub> to <i>n</i>-C<sub>28</sub>), internally branched monomethylalkanes at carbons 3, 11, or 13, and dimethylalkanes were identified, which showed no qualitative differences in either sex and were similar in the samples with SPME fiber extraction and those with hexane extraction. The bioassay showed that 11-methylpentacosane (11-MeC<sub>25</sub>), 11-methylhexacosane (11-MeC<sub>26</sub>), and 11-methylheptacosane (11-MeC<sub>27</sub>) have sex-specific recognition functions that triggered more mating attempts at a female-specific ratio of 100:4:60 than at a male-specific ratio of 100:85:50. In addition, the female-specific ratio of 11-methylalkanes can elicit about 70% of male mating attempts within about 60 s, whereas live females elicit about 98% of male mating attempts within 25 s. The discrepancy in the initiation of mating attempts by synthetic mixtures and live females suggests that the methyl isomers 3-MeC<sub>25</sub>, 3-MeC<sub>27</sub>, and/or 11,15-diMeC<sub>27</sub> may also be involved in the mating behavior of <i>S. bifasciatus</i>. These results suggest that 11-MeC<sub>25</sub>, 11-MeC<sub>26</sub>, and 11-MeC<sub>27</sub> constitute the contact sex pheromone of <i>S. bifasciatus</i>, with the presence or absence of 11-MeC<sub>26</sub> in particular playing an important role in mate recognition by males.</p>\",\"PeriodicalId\":17087,\"journal\":{\"name\":\"Journal of Systematics and Evolution\",\"volume\":\"61 3\",\"pages\":\"498-505\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2023-01-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jse.12947\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Systematics and Evolution\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jse.12947\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Systematics and Evolution","FirstCategoryId":"1089","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jse.12947","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Solid-phase microextraction and cuticular hydrocarbon differences related to reproductive activity in juniper bark borer Semanotus bifasciatus Motschulsky
Cuticular hydrocarbons of Cerambycidae species can function as signals for sex recognition. Little is known about the copulatory signals of the juniper bark borer Semanotus bifasciatus, a major economic threat to Platycladus orientalis Franco in China. Here, we investigated the cuticular hydrocarbons of both sexes of S. bifasciatus to determine the chemically mediated mating signals using the solid-phase microextraction (SPME) technique with carbowax/divinylbenzene fibers (CAR/DVB) and then analyzed by coupled gas chromatography-mass spectrometry (GC-MS). A series of aliphatic saturated straight-chain n-alkanes (n-C23 to n-C28), internally branched monomethylalkanes at carbons 3, 11, or 13, and dimethylalkanes were identified, which showed no qualitative differences in either sex and were similar in the samples with SPME fiber extraction and those with hexane extraction. The bioassay showed that 11-methylpentacosane (11-MeC25), 11-methylhexacosane (11-MeC26), and 11-methylheptacosane (11-MeC27) have sex-specific recognition functions that triggered more mating attempts at a female-specific ratio of 100:4:60 than at a male-specific ratio of 100:85:50. In addition, the female-specific ratio of 11-methylalkanes can elicit about 70% of male mating attempts within about 60 s, whereas live females elicit about 98% of male mating attempts within 25 s. The discrepancy in the initiation of mating attempts by synthetic mixtures and live females suggests that the methyl isomers 3-MeC25, 3-MeC27, and/or 11,15-diMeC27 may also be involved in the mating behavior of S. bifasciatus. These results suggest that 11-MeC25, 11-MeC26, and 11-MeC27 constitute the contact sex pheromone of S. bifasciatus, with the presence or absence of 11-MeC26 in particular playing an important role in mate recognition by males.
期刊介绍:
Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.