固相微萃取和表皮碳氢化合物差异与刺柏二孔虫繁殖活性的关系

IF 3.7 1区 生物学 Q1 Agricultural and Biological Sciences Journal of Systematics and Evolution Pub Date : 2023-01-11 DOI:10.1111/jse.12947
Jia-Xing Fang, Da-Feng Chen, Xia Shi, Su-Fang Zhang, Fu Liu, Wei-Xing Shen, Chun-Yan Jia, Shen-Cheng Ma, Zhen Zhang, Xiang-Bo Kong
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引用次数: 1

摘要

Cerambycidae物种的表皮碳氢化合物可以作为性别识别的信号。对刺柏二孔虫的交配信号知之甚少,这是对中国侧柏的主要经济威胁。在这里,我们用碳蜡/二乙烯基苯纤维(CAR/DVB)固相微萃取(SPME)技术研究了双腹肌两性的表皮碳氢化合物,以确定化学介导的交配信号,然后用气相色谱-质谱联用(GC-MS)分析。鉴定了一系列脂族饱和直链正构烷烃(n-C23至n-C28)、碳原子数为3、11或13的内支化单甲基烷烃和二甲基烷烃,它们在性别上都没有定性差异,并且在SPME纤维提取的样品和己烷提取的样品中相似。生物测定表明,11甲基五碳烷(11-MeC25)、11甲基六碳烷(11甲基C26)和11甲基七碳烷(11MeC27)具有性别特异性识别功能,在100:4:60的雌性特异性比率下比在100:85:50的雄性特异性比率时触发更多的交配尝试。此外,11种甲基烷烃的雌性特异性比例可以在大约60秒内引发大约70%的雄性交配尝试 s、 而活的雌性在25年内引发了约98%的雄性交配尝试 s.合成混合物和活雌性在开始交配尝试方面的差异表明,甲基异构体3-MeC25、3-MeC27和/或11,15-diMeC27也可能参与双腹足线虫的交配行为。这些结果表明,11-MeC25、11-MeC26和11-MeC27构成了双腹足线虫的接触性信息素,11-MeC26的存在或不存在尤其在雄性的配偶识别中起着重要作用。
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Solid-phase microextraction and cuticular hydrocarbon differences related to reproductive activity in juniper bark borer Semanotus bifasciatus Motschulsky

Cuticular hydrocarbons of Cerambycidae species can function as signals for sex recognition. Little is known about the copulatory signals of the juniper bark borer Semanotus bifasciatus, a major economic threat to Platycladus orientalis Franco in China. Here, we investigated the cuticular hydrocarbons of both sexes of S. bifasciatus to determine the chemically mediated mating signals using the solid-phase microextraction (SPME) technique with carbowax/divinylbenzene fibers (CAR/DVB) and then analyzed by coupled gas chromatography-mass spectrometry (GC-MS). A series of aliphatic saturated straight-chain n-alkanes (n-C23 to n-C28), internally branched monomethylalkanes at carbons 3, 11, or 13, and dimethylalkanes were identified, which showed no qualitative differences in either sex and were similar in the samples with SPME fiber extraction and those with hexane extraction. The bioassay showed that 11-methylpentacosane (11-MeC25), 11-methylhexacosane (11-MeC26), and 11-methylheptacosane (11-MeC27) have sex-specific recognition functions that triggered more mating attempts at a female-specific ratio of 100:4:60 than at a male-specific ratio of 100:85:50. In addition, the female-specific ratio of 11-methylalkanes can elicit about 70% of male mating attempts within about 60 s, whereas live females elicit about 98% of male mating attempts within 25 s. The discrepancy in the initiation of mating attempts by synthetic mixtures and live females suggests that the methyl isomers 3-MeC25, 3-MeC27, and/or 11,15-diMeC27 may also be involved in the mating behavior of S. bifasciatus. These results suggest that 11-MeC25, 11-MeC26, and 11-MeC27 constitute the contact sex pheromone of S. bifasciatus, with the presence or absence of 11-MeC26 in particular playing an important role in mate recognition by males.

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来源期刊
Journal of Systematics and Evolution
Journal of Systematics and Evolution Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
7.40
自引率
8.10%
发文量
1368
审稿时长
6-12 weeks
期刊介绍: Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.
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