Christian Richter, Katharina F. Hohmann, Sabrina Toews, Daniel Mathieu, Nadide Altincekic, Jasleen Kaur Bains, Oliver Binas, Betül Ceylan, Elke Duchardt-Ferner, Jan Ferner, Boris Fürtig, J. Tassilo Grün, Martin Hengesbach, Daniel Hymon, Hendrik R. A. Jonker, Bozana Knezic, Sophie M. Korn, Tom Landgraf, Frank Löhr, Stephen A. Peter, Dennis J. Pyper, Nusrat S. Qureshi, Andreas Schlundt, Robbin Schnieders, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Julia E. Weigand, Julia Wirmer-Bartoschek, Kerstin Witt, Jens Wöhnert, Harald Schwalbe, Anna Wacker
{"title":"从SARS-CoV-2的5'-UTR中对茎环SL1的1H, 13C和15N进行定位","authors":"Christian Richter, Katharina F. Hohmann, Sabrina Toews, Daniel Mathieu, Nadide Altincekic, Jasleen Kaur Bains, Oliver Binas, Betül Ceylan, Elke Duchardt-Ferner, Jan Ferner, Boris Fürtig, J. Tassilo Grün, Martin Hengesbach, Daniel Hymon, Hendrik R. A. Jonker, Bozana Knezic, Sophie M. Korn, Tom Landgraf, Frank Löhr, Stephen A. Peter, Dennis J. Pyper, Nusrat S. Qureshi, Andreas Schlundt, Robbin Schnieders, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Julia E. Weigand, Julia Wirmer-Bartoschek, Kerstin Witt, Jens Wöhnert, Harald Schwalbe, Anna Wacker","doi":"10.1007/s12104-021-10047-2","DOIUrl":null,"url":null,"abstract":"<div><p>The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous <i>cis</i>-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.</p></div>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":"15 2","pages":"467 - 474"},"PeriodicalIF":0.8000,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12104-021-10047-2","citationCount":"4","resultStr":"{\"title\":\"1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2\",\"authors\":\"Christian Richter, Katharina F. Hohmann, Sabrina Toews, Daniel Mathieu, Nadide Altincekic, Jasleen Kaur Bains, Oliver Binas, Betül Ceylan, Elke Duchardt-Ferner, Jan Ferner, Boris Fürtig, J. Tassilo Grün, Martin Hengesbach, Daniel Hymon, Hendrik R. A. Jonker, Bozana Knezic, Sophie M. Korn, Tom Landgraf, Frank Löhr, Stephen A. Peter, Dennis J. Pyper, Nusrat S. Qureshi, Andreas Schlundt, Robbin Schnieders, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Julia E. Weigand, Julia Wirmer-Bartoschek, Kerstin Witt, Jens Wöhnert, Harald Schwalbe, Anna Wacker\",\"doi\":\"10.1007/s12104-021-10047-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous <i>cis</i>-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.</p></div>\",\"PeriodicalId\":492,\"journal\":{\"name\":\"Biomolecular NMR Assignments\",\"volume\":\"15 2\",\"pages\":\"467 - 474\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2021-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s12104-021-10047-2\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomolecular NMR Assignments\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12104-021-10047-2\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecular NMR Assignments","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s12104-021-10047-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}
1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2
The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7–33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1H, 13C and 15N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.
期刊介绍：
Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties.
Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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