Bryan W. Lehner , Neil P. Schultes , Douglas W. Dingman
{"title":"不同毒力模式的芽孢杆菌幼虫亚种也根据营养生长特征和烯醇化酶同工酶生化特性进行分组","authors":"Bryan W. Lehner , Neil P. Schultes , Douglas W. Dingman","doi":"10.1016/j.aggene.2017.09.002","DOIUrl":null,"url":null,"abstract":"<div><p><span><em>Paenibacillus larvae</em></span><span><span><span> – the causal agent of American foul brood disease in Honey bees – group to different subspecies based upon disease progression and virulence as well as by molecular genotype. </span>Vegetative growth<span> studies reveal that virulence-grouped subspecies arrive at different saturated cell densities. In addition, strains segregating based upon virulence phenotype contain different genotypes in the locus encoding for the key glycolytic enzyme enolase. </span></span>DNA sequence comparison of enolase loci from 7 </span><em>Paenibacillus larvae</em><span> strains identified 6 single-nucleotide polymorphisms (SNP) that segregated based on subspecies virulence classification. Only one polymorphism represented a change in amino acid coding (glycine or alanine) at position 331 of the protein. The kinetic properties of two recombinant enolase proteins expressed from enolase alleles isolated from different virulence classed strains (</span><em>P. larvae</em> ATCC 9545 and SAG 10367) yielded a K<sub>m</sub> and of 4.2<!--> <!-->μM and 1.5<!--> <!-->μM and V<sub>max</sub> of 16.2<!--> <!-->μmol<!--> <!-->min<sup>−<!--> <!-->1</sup> <!-->mg<sup>−<!--> <!-->1</sup> and 10.8<!--> <!-->μmol<!--> <!-->min<sup>−<!--> <!-->1</sup> <!-->mg<sup>−<!--> <!-->1</sup>, respectively. Enolase from <em>P. larvae</em> SAG 10367 had a maximum reaction velocity lower than and a specificity constant approximately 1.6<!--> <!-->× higher than that of <em>P. larvae</em> ATCC 9545.</p></div>","PeriodicalId":37751,"journal":{"name":"Agri Gene","volume":"6 ","pages":"Pages 31-36"},"PeriodicalIF":0.0000,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aggene.2017.09.002","citationCount":"1","resultStr":"{\"title\":\"Paenibacillus larvae subspecies with dissimilar virulence patterns also group by vegetative growth characteristics and enolase isozyme biochemical properties\",\"authors\":\"Bryan W. Lehner , Neil P. Schultes , Douglas W. Dingman\",\"doi\":\"10.1016/j.aggene.2017.09.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><em>Paenibacillus larvae</em></span><span><span><span> – the causal agent of American foul brood disease in Honey bees – group to different subspecies based upon disease progression and virulence as well as by molecular genotype. </span>Vegetative growth<span> studies reveal that virulence-grouped subspecies arrive at different saturated cell densities. In addition, strains segregating based upon virulence phenotype contain different genotypes in the locus encoding for the key glycolytic enzyme enolase. </span></span>DNA sequence comparison of enolase loci from 7 </span><em>Paenibacillus larvae</em><span> strains identified 6 single-nucleotide polymorphisms (SNP) that segregated based on subspecies virulence classification. Only one polymorphism represented a change in amino acid coding (glycine or alanine) at position 331 of the protein. The kinetic properties of two recombinant enolase proteins expressed from enolase alleles isolated from different virulence classed strains (</span><em>P. larvae</em> ATCC 9545 and SAG 10367) yielded a K<sub>m</sub> and of 4.2<!--> <!-->μM and 1.5<!--> <!-->μM and V<sub>max</sub> of 16.2<!--> <!-->μmol<!--> <!-->min<sup>−<!--> <!-->1</sup> <!-->mg<sup>−<!--> <!-->1</sup> and 10.8<!--> <!-->μmol<!--> <!-->min<sup>−<!--> <!-->1</sup> <!-->mg<sup>−<!--> <!-->1</sup>, respectively. Enolase from <em>P. larvae</em> SAG 10367 had a maximum reaction velocity lower than and a specificity constant approximately 1.6<!--> <!-->× higher than that of <em>P. larvae</em> ATCC 9545.</p></div>\",\"PeriodicalId\":37751,\"journal\":{\"name\":\"Agri Gene\",\"volume\":\"6 \",\"pages\":\"Pages 31-36\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.aggene.2017.09.002\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agri Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352215117300181\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agri Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352215117300181","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Paenibacillus larvae subspecies with dissimilar virulence patterns also group by vegetative growth characteristics and enolase isozyme biochemical properties
Paenibacillus larvae – the causal agent of American foul brood disease in Honey bees – group to different subspecies based upon disease progression and virulence as well as by molecular genotype. Vegetative growth studies reveal that virulence-grouped subspecies arrive at different saturated cell densities. In addition, strains segregating based upon virulence phenotype contain different genotypes in the locus encoding for the key glycolytic enzyme enolase. DNA sequence comparison of enolase loci from 7 Paenibacillus larvae strains identified 6 single-nucleotide polymorphisms (SNP) that segregated based on subspecies virulence classification. Only one polymorphism represented a change in amino acid coding (glycine or alanine) at position 331 of the protein. The kinetic properties of two recombinant enolase proteins expressed from enolase alleles isolated from different virulence classed strains (P. larvae ATCC 9545 and SAG 10367) yielded a Km and of 4.2 μM and 1.5 μM and Vmax of 16.2 μmol min− 1 mg− 1 and 10.8 μmol min− 1 mg− 1, respectively. Enolase from P. larvae SAG 10367 had a maximum reaction velocity lower than and a specificity constant approximately 1.6 × higher than that of P. larvae ATCC 9545.
Agri GeneAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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