不同饲料类型下鱼油添加量及添加周期对荷斯坦公牛脂肪组织生成位点及脂肪代谢相关酶基因表达的影响

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2016-08-01 DOI:10.1016/j.aggene.2016.07.003
Hossein Zakariapour Bahnamiri , Mahdi Ganjkhanlou , Mostafa Sadeghi , Mohammad Jjavad Najaf-panah , Abolfazl Zali , Wang ZuYang
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引用次数: 1

摘要

为研究苜蓿干草(AH)比例、鱼油(FO)添加量和鱼油添加时间对不同脂肪组织库脂蛋白脂肪酶(LPL)、过氧化物酶体增殖物激活受体γ (PPARγ)和硬脂酰辅酶a去饱和酶(SCD)基因表达的影响,采用2 × 3因子设计,将36头初始体重345±61 kg的荷斯坦公牛随机分为6个饲粮处理。分别添加2个水平的水气(占饲粮干物质的10%和20%)和3个水平的鱼油(占饲粮干物质的0、1.05和2.1%)。在屠宰后的中期和后期(第90天)采集脂肪组织样本。采用RT-qPCR技术进行基因表达分析。脂质基因的相对mRNA丰度与AH比例和FO相互作用无关。添加鱼油增加了内脏和皮下脂肪组织中LPL和SCD基因的表达,与AH比例无关(P <0.05)。PPARγ的基因表达不受饮食处理的影响。添加鱼油对皮下组织SCD1基因mRNA丰度的影响大于内脏脂肪组织(P <0.01)。延长鱼油补充时间后,PPARγ和SCD酶的基因表达增加(P <0.01),而后期LPL mRNA丰度低于中期(P <0.01)。结果表明,添加鱼油增加了脂肪组织SCD mRNA的丰度,而在增加mRNA丰度和酶活性的情况下,UFAs浓度增加,有利于人类和动物的健康。此外,发现鱼油补充的持续时间是调节脂肪组织代谢相关酶的重要因素。
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Effects of fish oil supplementation and supplementation period on adipose tissue generation sites and the gene expression of enzymes involved in metabolizing adipose tissue in Holstein bulls under various forage types

To investigate the effects of alfalfa hay (AH) proportion, fish oil (FO) supplementation, and the length of FO supplementation on lipoprotein lipase (LPL), peroxisome proliferator activated receptorγ (PPARγ) and stearoyl-COA desaturase (SCD) gene expression in various adipose tissue depots, 36 Holstein bulls (initial body weight of 345 ± 61 kg) were randomly assigned to 6 dietary treatments in a 2 × 3 factorial arrangement, with 2 levels of AH (10 and 20% of dietary dry matter) and 3 levels of FO (0, 1.05 and 2.1% of dry matter). Samples of adipose tissue were collected at the middle and end of the period (day 90), after slaughtering. The RT-qPCR technique was used for gene expression analyses. The relative mRNA abundance of lipogenic genes was not correlated with AH proportion and FO interaction. Supplementation of FO increased LPL and SCD gene expression in visceral and subcutaneous adipose tissues regardless of AH proportion (P < 0.05). The gene expression of PPARγ was not affected by dietary treatments. Supplementation of FO increased the mRNA abundance of SCD1 gene more in subcutaneous than visceral adipose tissue (P < 0.01). Gene expression of PPARγ and SCD enzymes increased as a result of prolonged FO supplementation (P < 0.01), whereas LPL mRNA abundance was lower at the end of period comparing to the middle period (P < 0.01). The results demonstrate that FO supplementation increases the abundance of adipose tissue SCD mRNA, which in the case of enhancement in UFAs concentration as a result of higher mRNA abundance and enzyme activity, can benefit both human and animal health. Moreover, the duration of FO supplementation was found to be an important factor in regulating enzymes involved in adipose tissue metabolism.

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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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Editorial Board Evolution of the gene of the lectin, Phalera flavescens agglutinin (PFA) Expression of lipogenic genes in the muscle of beef cattle fed oilseeds and vitamin E FABP1 and SLC2A5 expression levels affect feed efficiency-related traits Characterization and expression analysis of a GnRH-like peptide in the Pacific abalone, Haliotis discus hannai
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