Hossein Zakariapour Bahnamiri , Mahdi Ganjkhanlou , Mostafa Sadeghi , Mohammad Jjavad Najaf-panah , Abolfazl Zali , Wang ZuYang
{"title":"不同饲料类型下鱼油添加量及添加周期对荷斯坦公牛脂肪组织生成位点及脂肪代谢相关酶基因表达的影响","authors":"Hossein Zakariapour Bahnamiri , Mahdi Ganjkhanlou , Mostafa Sadeghi , Mohammad Jjavad Najaf-panah , Abolfazl Zali , Wang ZuYang","doi":"10.1016/j.aggene.2016.07.003","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>To investigate the effects of alfalfa hay<span> (AH) proportion, fish oil (FO) supplementation, and the length of FO supplementation on lipoprotein lipase (LPL), peroxisome proliferator activated receptorγ (PPARγ) and stearoyl-COA desaturase (SCD) gene expression in various </span></span>adipose tissue<span> depots, 36 Holstein bulls (initial body weight of 345</span></span> <!-->±<!--> <!-->61<!--> <!-->kg) were randomly assigned to 6 dietary treatments in a 2<!--> <!-->×<!--> <span>3 factorial arrangement, with 2 levels of AH (10 and 20% of dietary dry matter) and 3 levels of FO (0, 1.05 and 2.1% of dry matter). Samples of adipose tissue were collected at the middle and end of the period (day 90), after slaughtering. The RT-qPCR technique was used for gene expression analyses. The relative mRNA abundance of lipogenic genes was not correlated with AH proportion and FO interaction. Supplementation of FO increased LPL and SCD gene expression in visceral and subcutaneous adipose tissues regardless of AH proportion (</span><em>P</em> <!--><<!--> <!-->0.05). The gene expression of PPARγ was not affected by dietary treatments. Supplementation of FO increased the mRNA abundance of SCD1 gene more in subcutaneous than visceral adipose tissue (<em>P</em> <!--><<!--> <!-->0.01). Gene expression of PPARγ and SCD enzymes increased as a result of prolonged FO supplementation (<em>P</em> <!--><<!--> <!-->0.01), whereas LPL mRNA abundance was lower at the end of period comparing to the middle period (<em>P</em> <!--><<!--> <span>0.01). The results demonstrate that FO supplementation increases the abundance of adipose tissue SCD mRNA, which in the case of enhancement in UFAs concentration as a result of higher mRNA abundance and enzyme activity, can benefit both human and animal health. Moreover, the duration of FO supplementation was found to be an important factor in regulating enzymes involved in adipose tissue metabolism.</span></p></div>","PeriodicalId":37751,"journal":{"name":"Agri Gene","volume":"1 ","pages":"Pages 72-78"},"PeriodicalIF":0.0000,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aggene.2016.07.003","citationCount":"1","resultStr":"{\"title\":\"Effects of fish oil supplementation and supplementation period on adipose tissue generation sites and the gene expression of enzymes involved in metabolizing adipose tissue in Holstein bulls under various forage types\",\"authors\":\"Hossein Zakariapour Bahnamiri , Mahdi Ganjkhanlou , Mostafa Sadeghi , Mohammad Jjavad Najaf-panah , Abolfazl Zali , Wang ZuYang\",\"doi\":\"10.1016/j.aggene.2016.07.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>To investigate the effects of alfalfa hay<span> (AH) proportion, fish oil (FO) supplementation, and the length of FO supplementation on lipoprotein lipase (LPL), peroxisome proliferator activated receptorγ (PPARγ) and stearoyl-COA desaturase (SCD) gene expression in various </span></span>adipose tissue<span> depots, 36 Holstein bulls (initial body weight of 345</span></span> <!-->±<!--> <!-->61<!--> <!-->kg) were randomly assigned to 6 dietary treatments in a 2<!--> <!-->×<!--> <span>3 factorial arrangement, with 2 levels of AH (10 and 20% of dietary dry matter) and 3 levels of FO (0, 1.05 and 2.1% of dry matter). Samples of adipose tissue were collected at the middle and end of the period (day 90), after slaughtering. The RT-qPCR technique was used for gene expression analyses. The relative mRNA abundance of lipogenic genes was not correlated with AH proportion and FO interaction. Supplementation of FO increased LPL and SCD gene expression in visceral and subcutaneous adipose tissues regardless of AH proportion (</span><em>P</em> <!--><<!--> <!-->0.05). The gene expression of PPARγ was not affected by dietary treatments. Supplementation of FO increased the mRNA abundance of SCD1 gene more in subcutaneous than visceral adipose tissue (<em>P</em> <!--><<!--> <!-->0.01). Gene expression of PPARγ and SCD enzymes increased as a result of prolonged FO supplementation (<em>P</em> <!--><<!--> <!-->0.01), whereas LPL mRNA abundance was lower at the end of period comparing to the middle period (<em>P</em> <!--><<!--> <span>0.01). The results demonstrate that FO supplementation increases the abundance of adipose tissue SCD mRNA, which in the case of enhancement in UFAs concentration as a result of higher mRNA abundance and enzyme activity, can benefit both human and animal health. Moreover, the duration of FO supplementation was found to be an important factor in regulating enzymes involved in adipose tissue metabolism.</span></p></div>\",\"PeriodicalId\":37751,\"journal\":{\"name\":\"Agri Gene\",\"volume\":\"1 \",\"pages\":\"Pages 72-78\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.aggene.2016.07.003\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agri Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352215116300137\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agri Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352215116300137","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Effects of fish oil supplementation and supplementation period on adipose tissue generation sites and the gene expression of enzymes involved in metabolizing adipose tissue in Holstein bulls under various forage types
To investigate the effects of alfalfa hay (AH) proportion, fish oil (FO) supplementation, and the length of FO supplementation on lipoprotein lipase (LPL), peroxisome proliferator activated receptorγ (PPARγ) and stearoyl-COA desaturase (SCD) gene expression in various adipose tissue depots, 36 Holstein bulls (initial body weight of 345 ± 61 kg) were randomly assigned to 6 dietary treatments in a 2 × 3 factorial arrangement, with 2 levels of AH (10 and 20% of dietary dry matter) and 3 levels of FO (0, 1.05 and 2.1% of dry matter). Samples of adipose tissue were collected at the middle and end of the period (day 90), after slaughtering. The RT-qPCR technique was used for gene expression analyses. The relative mRNA abundance of lipogenic genes was not correlated with AH proportion and FO interaction. Supplementation of FO increased LPL and SCD gene expression in visceral and subcutaneous adipose tissues regardless of AH proportion (P < 0.05). The gene expression of PPARγ was not affected by dietary treatments. Supplementation of FO increased the mRNA abundance of SCD1 gene more in subcutaneous than visceral adipose tissue (P < 0.01). Gene expression of PPARγ and SCD enzymes increased as a result of prolonged FO supplementation (P < 0.01), whereas LPL mRNA abundance was lower at the end of period comparing to the middle period (P < 0.01). The results demonstrate that FO supplementation increases the abundance of adipose tissue SCD mRNA, which in the case of enhancement in UFAs concentration as a result of higher mRNA abundance and enzyme activity, can benefit both human and animal health. Moreover, the duration of FO supplementation was found to be an important factor in regulating enzymes involved in adipose tissue metabolism.
Agri GeneAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.