不同牌子漂白剂处理外生菌根真菌微果菇的透性、细胞壁超微结构和担子孢子的萌发

IF 0.6 4区 农林科学 Q4 FORESTRY Revista Arvore Pub Date : 2021-07-02 DOI:10.1590/1806-908820210000009
M. Silvério, Jaqueline Maria do Nascimento, J. Serrão, M. Tótola, M. Costa
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引用次数: 0

摘要

摘要外生菌根真菌Pisolithus microcarpus的担子孢子具有不透水的细胞壁,这可能与这些繁殖体的发芽率低有关,这使得它们难以获得单核细胞并用于接种剂。本研究旨在探讨不同浓度的商品漂白剂对小槭树担子孢子通透性的影响,并分析其细胞壁超微结构、繁殖体活力和萌发能力的变化。在桉树人工林中采集了真菌担子孢子,并用不同的漂白剂浓度和暴露时间进行渗透。然后用扫描电镜和透射电镜对担子孢子进行分析。浸透性担子孢子的百分比随漂白剂的品牌、浓度和暴露时间的不同而变化。不同担子果孢子对漂白剂渗透处理的敏感性不同。在漂白剂浓度为15%和50%、暴露时间为40 s的条件下,观察到透性担子孢子超微结构的变化,表面发生变化,最外层壁的针状体丢失。经5%漂白剂渗透40 s后,80%的渗透孢子存活,这些繁殖体在citriodora存在下孵育15天后产生真菌菌落。然而,获得的萌发率为0.001 %,与未透性的担子孢子相似,这表明除了细胞壁透性外,还有其他因素对萌发过程起决定作用。
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PERMEABILIZATION, CELL WALL ULTRASTRUCTURE, AND GERMINATION OF BASIDIOSPORES OF THE ECTOMYCORRHIZAL FUNGUS Pisolithus microcarpus TREATED WITH DIFFERENT COMMERCIAL BRANDS OF BLEACH
ABSTRACT Basidiospores of the ectomycorrhizal fungus Pisolithus microcarpus have an impermeable cell wall, a characteristic that is possibly related to the low germination percentages of these propagules, which makes it difficult to obtain monokaryons and use these spores in inoculants. The objective of this study was to evaluate the effect of different concentrations of commercial bleach on the permeabilization of P. microcarpus basidiospores and to analyze the alterations caused in the cell wall ultrastructure and the viability and germination capacity of these propagules. Fungal basidiospores were collected in eucalyptus plantations and permeabilized using different bleach concentrations and exposure times. The basidiospores were then analyzed by scanning and transmission electron microscopy. The percentage of permeabilized basidiospores varied with the commercial brand, bleach concentration, and exposure time. Basidiospores of different basidiocarps differed in susceptibility to permeabilization treatment with bleach. Changes in the ultrastructure of permeabilized basidiospores were observed at bleach concentrations of 15 and 50 % for an exposure time of 40 s, with surface changes and loss of the spicules of the outermost layer of the wall. After permeabilization with 5 % bleach for 40 s, 80 % of the permeabilized spores were viable, resulting in the production of fungal colonies after 15 days of incubation of these propagules in the presence of Corymbia citriodora. However, the germination percentage obtained, 0.001 %, was similar to that of non-permeabilized basidiospores, indicating that other factors, besides cell wall permeability, are determinant for the germination process.
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来源期刊
Revista Arvore
Revista Arvore FORESTRY-
CiteScore
1.00
自引率
0.00%
发文量
32
审稿时长
4-8 weeks
期刊介绍: A Revista Árvore é um veículo de comunicação científica da Sociedade de Investigações Florestais – SIF. O jornal é de acesso gratuito, revisado por pares, que publica bimestralmente trabalhos científicos originais no campo da Ciência Florestal. As áreas temáticas para publicação são: Ambiência e Conservação da Natureza, Manejo Florestal, Silvicultura e Tecnologia da Madeira e Utilização de Produtos Florestais. A política editorial visa manter alta conduta ética em relação à publicação e aos seus funcionários, rigor na qualidade dos artigos científicos, seleção de revisores qualificados, respeito profissional aos autores e processo de tomada de decisão imparcial. A Revista Árvore publica artigos apenas em inglês. Artigos de revisão podem ser publicados se houver uma discussão relevante resumindo o estado da arte sobre o assunto. A revisão estrita da literatura não é aceita.
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