摘要巴西山核桃树(山核桃)的茎溃疡病是由BOTRYOSPHAERIACEAE真菌引起的

IF 0.6 4区 农林科学 Q4 FORESTRY Revista Arvore Pub Date : 2022-01-01 DOI:10.1590/1806-908820220000015
J. M. Rolim, J. E. Rabuske, L. G. Savian, C. Walker, J. S. Sarzi, J. C. P. Silva, M. Muniz
{"title":"摘要巴西山核桃树(山核桃)的茎溃疡病是由BOTRYOSPHAERIACEAE真菌引起的","authors":"J. M. Rolim, J. E. Rabuske, L. G. Savian, C. Walker, J. S. Sarzi, J. C. P. Silva, M. Muniz","doi":"10.1590/1806-908820220000015","DOIUrl":null,"url":null,"abstract":"ABSTRACT Pecan tree [Carya illinoinensis (Wangenh.) K. Koch] is a widespread species in the southern region of Brazil and commercially important for nut and wood production. Stem cankers in pecan trees negatively impact the orchard, but the biotic causes of cankers are usually not identified. Symptoms of stem canker were observed in about 60% of pecan trees from orchards in the states of Rio Grande do Sul and Paraná. Therefore, this study aimed to analyze the molecular and morphophysiological characteristics of fungi associated with pecan canker in orchards in southern Brazil and to confirm their pathogenicity in pecan seedlings. Samples from symptomatic stems were collected in different municipalities and possible causal agents were isolated. Molecular identification was performed by the Polymerase Chain Reaction technique for amplification of the EF-1α region and subsequent sequencing. The sequencing, followed by morphophysiological aspects of mycelial growth and colony pigmentation, allowed the identification of a species complex included in the Botryosphaeriaceae family. The isolates were classified as Lasiodiplodia theobromae, Pseudofusicoccum kimberleyense and Neofusicoccum parvum. Pathogenicity was tested by inoculating the isolates into pecan seedlings to evaluate the symptoms. All isolates caused canker in the stem, but there was variation in the severity. Therefore, different species of the Botryosphaeriaceae family are responsible for stem canker occurrence in pecan trees in the southern region of Brazil, but at different levels of injury. Thus, it is crucial to identify and understand the behavior of the fungal isolates to best control cankers in pecan orchards.","PeriodicalId":21214,"journal":{"name":"Revista Arvore","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"FUNGI OF THE BOTRYOSPHAERIACEAE FAMILY CAUSE DIFFERENT LEVELS OF STEM CANKER ON PECAN TREES (Carya illinoinensis) IN BRAZIL\",\"authors\":\"J. M. Rolim, J. E. Rabuske, L. G. Savian, C. Walker, J. S. Sarzi, J. C. P. Silva, M. Muniz\",\"doi\":\"10.1590/1806-908820220000015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Pecan tree [Carya illinoinensis (Wangenh.) K. Koch] is a widespread species in the southern region of Brazil and commercially important for nut and wood production. Stem cankers in pecan trees negatively impact the orchard, but the biotic causes of cankers are usually not identified. Symptoms of stem canker were observed in about 60% of pecan trees from orchards in the states of Rio Grande do Sul and Paraná. Therefore, this study aimed to analyze the molecular and morphophysiological characteristics of fungi associated with pecan canker in orchards in southern Brazil and to confirm their pathogenicity in pecan seedlings. Samples from symptomatic stems were collected in different municipalities and possible causal agents were isolated. Molecular identification was performed by the Polymerase Chain Reaction technique for amplification of the EF-1α region and subsequent sequencing. The sequencing, followed by morphophysiological aspects of mycelial growth and colony pigmentation, allowed the identification of a species complex included in the Botryosphaeriaceae family. The isolates were classified as Lasiodiplodia theobromae, Pseudofusicoccum kimberleyense and Neofusicoccum parvum. Pathogenicity was tested by inoculating the isolates into pecan seedlings to evaluate the symptoms. All isolates caused canker in the stem, but there was variation in the severity. Therefore, different species of the Botryosphaeriaceae family are responsible for stem canker occurrence in pecan trees in the southern region of Brazil, but at different levels of injury. Thus, it is crucial to identify and understand the behavior of the fungal isolates to best control cankers in pecan orchards.\",\"PeriodicalId\":21214,\"journal\":{\"name\":\"Revista Arvore\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Arvore\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1590/1806-908820220000015\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Arvore","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1806-908820220000015","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 2

摘要

山核桃[Carya illinensis (Wangenh.)][K. Koch]是巴西南部地区广泛分布的树种,对坚果和木材生产具有重要的商业价值。核桃树的茎溃疡病对果园有负面影响,但引起溃疡病的生物原因通常不确定。在巴西南大德州和帕拉纳州的果园中,大约60%的山核桃树出现了茎溃疡病的症状。因此,本研究旨在分析巴西南部果园山核桃溃疡病相关真菌的分子和形态生理特征,并确认其在山核桃幼苗中的致病性。在不同的城市采集了有症状的茎标本,并分离出可能的病原。采用聚合酶链反应技术进行分子鉴定,扩增EF-1α区域并进行测序。测序,其次是菌丝生长和菌落色素沉着的形态生理方面,允许鉴定一个物种复合体,包括在Botryosphaeriaceae家族。分离物分类为:甘蔗Lasiodiplodia theobromae、金伯利Pseudofusicoccum kimberleyense和Neofusicoccum parum。通过将分离株接种到山核桃幼苗上进行致病性试验,评价其症状。所有菌株都引起茎部溃疡病,但严重程度有所不同。因此,巴西南部地区山核桃发生茎溃疡病的原因是不同种的Botryosphaeriaceae,但其伤害程度不同。因此,鉴定和了解真菌分离株的行为是控制山核桃果园溃疡病的关键。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
FUNGI OF THE BOTRYOSPHAERIACEAE FAMILY CAUSE DIFFERENT LEVELS OF STEM CANKER ON PECAN TREES (Carya illinoinensis) IN BRAZIL
ABSTRACT Pecan tree [Carya illinoinensis (Wangenh.) K. Koch] is a widespread species in the southern region of Brazil and commercially important for nut and wood production. Stem cankers in pecan trees negatively impact the orchard, but the biotic causes of cankers are usually not identified. Symptoms of stem canker were observed in about 60% of pecan trees from orchards in the states of Rio Grande do Sul and Paraná. Therefore, this study aimed to analyze the molecular and morphophysiological characteristics of fungi associated with pecan canker in orchards in southern Brazil and to confirm their pathogenicity in pecan seedlings. Samples from symptomatic stems were collected in different municipalities and possible causal agents were isolated. Molecular identification was performed by the Polymerase Chain Reaction technique for amplification of the EF-1α region and subsequent sequencing. The sequencing, followed by morphophysiological aspects of mycelial growth and colony pigmentation, allowed the identification of a species complex included in the Botryosphaeriaceae family. The isolates were classified as Lasiodiplodia theobromae, Pseudofusicoccum kimberleyense and Neofusicoccum parvum. Pathogenicity was tested by inoculating the isolates into pecan seedlings to evaluate the symptoms. All isolates caused canker in the stem, but there was variation in the severity. Therefore, different species of the Botryosphaeriaceae family are responsible for stem canker occurrence in pecan trees in the southern region of Brazil, but at different levels of injury. Thus, it is crucial to identify and understand the behavior of the fungal isolates to best control cankers in pecan orchards.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Revista Arvore
Revista Arvore FORESTRY-
CiteScore
1.00
自引率
0.00%
发文量
32
审稿时长
4-8 weeks
期刊介绍: A Revista Árvore é um veículo de comunicação científica da Sociedade de Investigações Florestais – SIF. O jornal é de acesso gratuito, revisado por pares, que publica bimestralmente trabalhos científicos originais no campo da Ciência Florestal. As áreas temáticas para publicação são: Ambiência e Conservação da Natureza, Manejo Florestal, Silvicultura e Tecnologia da Madeira e Utilização de Produtos Florestais. A política editorial visa manter alta conduta ética em relação à publicação e aos seus funcionários, rigor na qualidade dos artigos científicos, seleção de revisores qualificados, respeito profissional aos autores e processo de tomada de decisão imparcial. A Revista Árvore publica artigos apenas em inglês. Artigos de revisão podem ser publicados se houver uma discussão relevante resumindo o estado da arte sobre o assunto. A revisão estrita da literatura não é aceita.
期刊最新文献
MIXED MODELS FOR NUTRIENTS PREDICTION IN SPECIES OF THE BRAZILIAN CAATINGA BIOME EMERGENCE, INITIAL GROWTH, AND SEEDLING QUALITY OF Eremanthus incanus: SUBSIDIES FOR GENETIC BREEDING AND CONSERVATION VEGETATIVE RESCUE AND PROPAGATION OF NATIVE Ilex paraguariensis POPULATIONS IN SANTA CATARINA STATE, BRAZIL EFFECT OF pH ON THE ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF WOOD VINEGAR (PYROLIGNEOUS EXTRACT) FROM EUCALYPTUS CHARACTERISTIC STRENGTHS IN THE COMPRESSION AND IN THE STATIC BENDING AS PARAMETERS TO ESTIMATE CHARACTERISTIC SHEAR STRENGTH FOR TIMBER DESIGN
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1