{"title":"模拟微重力条件下注射必需磷脂后小鼠卵母细胞细胞骨架蛋白含量的变化","authors":"М.А. Sventitskaya, I. V. Ogneva","doi":"10.21687/0233-528x-2023-57-1-44-48","DOIUrl":null,"url":null,"abstract":"The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CHANGE IN THE CONTENT OF CYTOSKELETAL PROTEINS IN MOUSE OOCYTES AFTER INJECTION OF ESSENTIAL PHOSPHOLIPIDS IN THE CONDITION OF MODELED MICROGRAVITY\",\"authors\":\"М.А. Sventitskaya, I. V. Ogneva\",\"doi\":\"10.21687/0233-528x-2023-57-1-44-48\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.\",\"PeriodicalId\":8683,\"journal\":{\"name\":\"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21687/0233-528x-2023-57-1-44-48\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21687/0233-528x-2023-57-1-44-48","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
CHANGE IN THE CONTENT OF CYTOSKELETAL PROTEINS IN MOUSE OOCYTES AFTER INJECTION OF ESSENTIAL PHOSPHOLIPIDS IN THE CONDITION OF MODELED MICROGRAVITY
The paper presents evaluation of the effect of different doses of essential phospholipids on the cytoskeleton of mouse oocyte microtubes and microfilaments cultivated on a microgravity simulator. Total content of acetylated alpha-tubulin and beta-actin was determined by immunocytochemistry. After the cultivation in modeled microgravity, the relative content of acetylated alpha-tubulin was decreased, whereas the beta-actin level remained unchanged. Injection of essential phospholipids (350 mg/kg of body mass) caused recovery of this parameter in the conditions reproducing the effects of microgravity.
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