Oxidative Stress and the Antioxidant Capacity of Plasma in Patients with Helicobacter pylori -Positive Gastroduodenitis and the Effect on Serum Iron Levels

Aim: To evaluate the oxidative stress and antioxidant protection in patients with chronic Gastroduodenitis and the relationship between their levels with the etiologic agent H. pylori and how this affects iron homeostasis. Introduction: Several records indicate that oxidative stress plays a significant role in the pathogenesis and progression of chronic inflammatory diseases of the gastrointestinal tract. H. pylori infection generates free oxygen and nitrogen species (ROS/RNS) cause damage to the gastric mucosa. Materials and Methods: 55 patients with gastritis and 80 healthy volunteers were studied, with a mean age of 59.69±11.52 and 53.8 ±0.8 years, respectively. Both groups define routine laboratory parameters (including CRP and Iron). Serum levels of antibodies against H. pylori (ELISA DiaMetra Italy) and antigenic presence in faecal matter were examined. Patients undergo endoscopic examination. Oxidative stress (dROMs) and serum antioxidant capacity (BAP) were determined spectrophotometrically on a semi-automatic Carpe diam analyzer. (Diacron Labs Italy). Statistical methods used: T-test for comparison of mean values, descriptive and correlation analysis. Results: The patient group showed significantly elevated mean dROMs values compared to the control group. Levels in patients with H. pylori infection differ significantly from HP negative ones. The BAP test showed a significant decrease compared to the control group. A similar relationship exists between the two patient groups for the BAP test. A moderate positive correlation between CRP and dROMs was found, and a moderate negative correlation with the BAP test. Increasing dROMs and decreasing BAP results in a decrease in serum iron levels. Conclusion: Determination of oxidative stress levels, antioxidant protection, and iron homeostasis may be used in the monitoring of patients with chronic Gastroduodenitis.