B. Laribi, N. Zoghlami, M. Lamine, K. Kouki, A. Ghorbel, A. Mougou
{"title":"基于rapd的一年生香菜居群遗传多样性评价","authors":"B. Laribi, N. Zoghlami, M. Lamine, K. Kouki, A. Ghorbel, A. Mougou","doi":"10.5053/EJOBIOS.2011.5.0.5","DOIUrl":null,"url":null,"abstract":"For the first time, genetic variability and differentiation among five annual caraway (Carum carvi) populations originating from Tunisia, Germany and Egypt were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity, population structure and gene flow. Fourteen primers generated a total of 136 discernible and reproducible bands across the analyzed populations, out of which 56 were polymorphic. The UPGMA cluster analysis permitted the discrimination of all the genotypes and their sorting into 3 main groups. Tunisian caraway populations diverged significantly from German and Egyptian ones. Population clustering was made dependently from geographic origin. This has been further explained at the DNA level as we were able to select a set of RAPD fingerprints unique to each of the studied populations. Furthermore, dimensional graph derived from factorial analysis of RAPD frequency data, allowed significant grouping of the genotypes into five sub-plots, representing each one population. Shannon's index values showed that variation ranks between rather than within populations. These results indicated that considerable genetic differences among C. carvi populations were registered.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2011-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":"{\"title\":\"RAPD-based assessment of genetic diversity among annual caraway (Carum carvi) populations\",\"authors\":\"B. Laribi, N. Zoghlami, M. Lamine, K. Kouki, A. Ghorbel, A. Mougou\",\"doi\":\"10.5053/EJOBIOS.2011.5.0.5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"For the first time, genetic variability and differentiation among five annual caraway (Carum carvi) populations originating from Tunisia, Germany and Egypt were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity, population structure and gene flow. Fourteen primers generated a total of 136 discernible and reproducible bands across the analyzed populations, out of which 56 were polymorphic. The UPGMA cluster analysis permitted the discrimination of all the genotypes and their sorting into 3 main groups. Tunisian caraway populations diverged significantly from German and Egyptian ones. Population clustering was made dependently from geographic origin. This has been further explained at the DNA level as we were able to select a set of RAPD fingerprints unique to each of the studied populations. Furthermore, dimensional graph derived from factorial analysis of RAPD frequency data, allowed significant grouping of the genotypes into five sub-plots, representing each one population. Shannon's index values showed that variation ranks between rather than within populations. These results indicated that considerable genetic differences among C. carvi populations were registered.\",\"PeriodicalId\":11848,\"journal\":{\"name\":\"Eurasian Journal of Biosciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eurasian Journal of Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5053/EJOBIOS.2011.5.0.5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eurasian Journal of Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5053/EJOBIOS.2011.5.0.5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
RAPD-based assessment of genetic diversity among annual caraway (Carum carvi) populations
For the first time, genetic variability and differentiation among five annual caraway (Carum carvi) populations originating from Tunisia, Germany and Egypt were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity, population structure and gene flow. Fourteen primers generated a total of 136 discernible and reproducible bands across the analyzed populations, out of which 56 were polymorphic. The UPGMA cluster analysis permitted the discrimination of all the genotypes and their sorting into 3 main groups. Tunisian caraway populations diverged significantly from German and Egyptian ones. Population clustering was made dependently from geographic origin. This has been further explained at the DNA level as we were able to select a set of RAPD fingerprints unique to each of the studied populations. Furthermore, dimensional graph derived from factorial analysis of RAPD frequency data, allowed significant grouping of the genotypes into five sub-plots, representing each one population. Shannon's index values showed that variation ranks between rather than within populations. These results indicated that considerable genetic differences among C. carvi populations were registered.
期刊介绍:
EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology