基于rapd的一年生香菜居群遗传多样性评价

Q3 Agricultural and Biological Sciences Eurasian Journal of Biosciences Pub Date : 2011-07-01 DOI:10.5053/EJOBIOS.2011.5.0.5
B. Laribi, N. Zoghlami, M. Lamine, K. Kouki, A. Ghorbel, A. Mougou
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引用次数: 11

摘要

首次对产自突尼斯、德国和埃及的5个一年生香菜(Carum carvi)居群的遗传变异和分化进行了研究。获得随机扩增多态性DNA (RAPD)标记数据,并对其遗传多样性、群体结构和基因流进行分析。14条引物在分析群体中共产生136条可识别和可复制的条带,其中56条是多态性的。UPGMA聚类分析可对所有基因型进行区分,并将其分为3个主要类群。突尼斯的香菜种群与德国和埃及的明显不同。种群聚类依赖于地理来源。这在DNA水平上得到了进一步的解释,因为我们能够为每个研究人群选择一组独特的RAPD指纹。此外,从RAPD频率数据的析因分析得出的维度图允许将基因型显著分组为五个子图,代表每个群体。香农的指数值表明,变异是在种群之间而不是种群内部进行排序的。这些结果表明,鲤科植物居群间存在较大的遗传差异。
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RAPD-based assessment of genetic diversity among annual caraway (Carum carvi) populations
For the first time, genetic variability and differentiation among five annual caraway (Carum carvi) populations originating from Tunisia, Germany and Egypt were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity, population structure and gene flow. Fourteen primers generated a total of 136 discernible and reproducible bands across the analyzed populations, out of which 56 were polymorphic. The UPGMA cluster analysis permitted the discrimination of all the genotypes and their sorting into 3 main groups. Tunisian caraway populations diverged significantly from German and Egyptian ones. Population clustering was made dependently from geographic origin. This has been further explained at the DNA level as we were able to select a set of RAPD fingerprints unique to each of the studied populations. Furthermore, dimensional graph derived from factorial analysis of RAPD frequency data, allowed significant grouping of the genotypes into five sub-plots, representing each one population. Shannon's index values showed that variation ranks between rather than within populations. These results indicated that considerable genetic differences among C. carvi populations were registered.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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