共轭亚油酸对肥胖的影响及其与巨噬细胞招募的关系:实验和免疫组织化学研究。

Q3 Medicine Journal of Microscopy and Ultrastructure Pub Date : 2022-11-14 eCollection Date: 2024-07-01 DOI:10.4103/jmau.jmau_25_22
Asmaa Gaber Abdou, Mohamed AbdElfattah Bendary, Sara E Abdou, Ghada S Amer
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引用次数: 0

摘要

背景:共轭亚油酸(CLA)在人类和动物身上已被证明具有抗脂肪作用。本研究旨在使用针对 CD68 和 CD 163 的免疫组化方法评估 CLA 的预防和治疗作用及其对招募的巨噬细胞类型的影响:40只当地品系成年雄性白化大鼠,分为对照组、CLA补充组、肥胖组、CLA预防性肥胖组和CLA治疗肥胖组。从研究组的内脏脂肪中获取活组织切片,评估组织病理学变化,并对 CD68 和 CD163 进行免疫组化染色:结果:与其他组相比,肥胖组脂肪细胞肥大,并有炎症细胞浸润。与对照组和补充 CLA 组相比,肥胖组的 CD68 阳性率明显升高。与肥胖组相比,CLA预防组和CLA处理组表现出轻微的免疫反应,CD68阳性率显著下降。与对照组和补充 CLA 组相比,肥胖组的 CD163 阳性率显著下降:结论:肥胖者脂肪组织的炎症特征是 M1 型巨噬细胞多于 M2 型巨噬细胞。结论:肥胖者的脂肪组织具有炎症特征,其中 M1 型巨噬细胞多于 M2 型巨噬细胞。CLA 可将招募的巨噬细胞导向抗炎亚型(M2),从而促进其在预防肥胖方面的有益作用。
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Impact of Conjugated Linoleic Acid on Obesity and Its Association with Macrophage Recruitment: Experimental and Immunohistochemical Study.

Background: Conjugated linoleic acid (CLA) has been shown in humans and animals to have anti-adipose effects. The current study aims to assess the prophylactic and therapeutic impact of CLA and its effect on recruited macrophage type using immunohistochemistry against CD68 and CD 163.

Materials and methods: Forty adult male albino rats of local strain were included in the study and divided into control, CLA-supplemented, obese, CLA-prophylactic obese, and CLA-treated obese groups. Biopsies from visceral fat of the investigated groups were obtained and assessed for histopathological changes and immunohistochemical staining for CD68 and CD163.

Results: Obese group showed hypertrophied adipocytes and infiltration by inflammatory cells compared to other groups. The obese group showed a marked increase in the CD68 positivity compared with that in the control and CLA-supplemented groups. CLA-prophylactic and CLA-treated groups showed mild immune reaction with a significant decrease in CD68 positivity compared to the obese group. The obese group showed a significant decrease in the CD163 positivity compared with that in the control and CLA-supplemented groups.

Conclusions: Adipose tissue in obese is characterized by inflammation with more M1 than M2 macrophages. CLA could direct the recruited macrophages toward the anti-inflammatory subtype (M2) which encourages its beneficial effects in prophylaxis from obesity.

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