应用多重pcr方法快速鉴定COVID-19大流行期间呼吸道感染病原

Q4 Medicine Medicinski Casopis Pub Date : 2022-01-01 DOI:10.5937/mckg56-41198
Sanja Zornic, Ivana Petrović, Bojana Luković, Jelena D Zivadinovic, Jelena Arsić, Lazar Bezarević, Zorana Đorđević
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引用次数: 0

摘要

目标。大多数呼吸道感染都有类似的症状,因此临床上很难确定其病因。本研究旨在展示分子诊断在识别呼吸道感染病原方面的重要性,特别是在2019冠状病毒病(COVID-19)大流行期间。方法。采用自动多重聚合酶链反应(PCR)检测,对2022年1月1日至8月1日期间在克拉古耶瓦茨大学临床中心住院的患者共849份样本进行了检测。使用BioFire-FilmArray-Respiratory Panel 2.1检测742份鼻咽拭子[鉴定19种病毒(包括SARS-CoV-2)和4种细菌],使用BioFire-FilmArray-Pneumonia Panel[鉴定18种细菌和9种病毒](biomac - rieux, Marcy l'Etoile,法国)检测107份气管吸入物。测试是按照制造商的说明进行的,结果在一小时内就出来了。结果。在582份(78.4%)样本中,BioFire-FilmArray-Respiratory Panel 2.1 plus检测至少鉴定出一种病原体。鼻病毒(20.6%)、SARS-CoV-2(17.7%)、甲型流感(17.5%)、呼吸道合胞病毒(12.4%)和副流感3(10.1%)最为常见。其他病毒的发现频率较低,在一个样本中检测到副百日咳博德泰拉。在85个(79.4%)样本中,biofire - filarray - pneumonia Panel试验至少鉴定出一种细菌或病毒。最常见的细菌是金黄色葡萄球菌(42.4%)、流感嗜血杆菌(41.2%)、肺炎链球菌(36.5%)、卡他莫拉菌(22.3%)和嗜肺军团菌(2.4%)。病毒中检出鼻病毒(36.5%)、腺病毒(23.5%)、甲型流感(11.8%)和冠状病毒(4.7%)。结论。多重pcr检测改善了治疗和流行病学措施的实施,防止了COVID-19感染和军团病的传播。
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Rapid dentification of etiological agents of respiratory tract infections during the COVID-19 pandemic using the multiplex-PCR method
Objective. Most respiratory infections have similar symptoms, so it is clinically difficult to determine their etiology. This study aimed to show the importance of molecular diagnostics in identifying the etiological agent of respiratory infections, especially during the coronavirus disease 2019 (COVID-19) pandemic. Methods. A total of 849 samples from patients hospitalized at the University Clinical Center Kragujevac (from January 1 to August 1, 2022) were examined using automated multiplex-polymerase chain reaction (PCR) tests. The BioFire-FilmArray-Respiratory Panel 2.1 test was used for 742 nasopharyngeal swabs [identification of 19 viruses (including SARS-CoV-2) and four bacteria], while the BioFire-FilmArray-Pneumonia Panel was used [identification of 18 bacteria and nine viruses] (BioMérieux, Marcy l'Etoile, France) for 107 tracheal aspirates. The tests were performed according to the manufacturer's instructions, and the results were available within an hour. Results. In 582 (78.4%) samples, the BioFire-FilmArray-Respiratory Panel 2.1 plus test identified at least one pathogen. The rhinovirus (20.6%), SARS-CoV-2 (17.7%), influenza A (17.5%), respiratory syncytial virus (12.4%), and parainfluenza 3 (10.1%) were the most common. Other viruses were found less frequently, and Bordetella parapertussis was detected in one sample. In 85 (79.4%) samples, the BioFire-FilmArray-Pneumonia Panel test identified at least one bacterium or virus. The most prevalent bacteria were Staphylococcus aureus (42.4%), Haemophilus influenzae (41.2%), Streptococcus pneumoniae (36.5%), Moraxella catarrhalis (22.3%), and Legionella pneumophila (2.4%). Among viruses, rhinovirus (36.5%), adenovirus (23.5%), influenza A (11.8%), and the genus Coronavirus (4.7%), were detected. Conclusion. Multiplex-PCR tests improved the implementation of therapeutic and epidemiological measures, preventing the spread of the COVID-19 infection and Legionnaires' disease.
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Medicinski Casopis
Medicinski Casopis Medicine-Medicine (all)
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